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Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors
EC Wheeler, AQ Vu, JM Einstein, M DiSalvo… - Nature …, 2020 - nature.com
Genetic screens using pooled CRISPR-based approaches are scalable and inexpensive,
but restricted to standard readouts, including survival, proliferation and sortable markers …
but restricted to standard readouts, including survival, proliferation and sortable markers …
A technology of a different sort: microraft arrays
B Cortés-Llanos, Y Wang, CE Sims, NL Allbritton - Lab on a Chip, 2021 - pubs.rsc.org
A common procedure performed throughout biomedical research is the selection and
isolation of biological entities such as organelles, cells and organoids from a mixed …
isolation of biological entities such as organelles, cells and organoids from a mixed …
Injection molded microfluidics for establishing high-density single cell arrays in an open hydrogel format
Here, we develop an injection molded microfluidic approach for single cell analysis by
making use of (1) rapidly curing injectable hydrogels,(2) a high density microfluidic weir trap …
making use of (1) rapidly curing injectable hydrogels,(2) a high density microfluidic weir trap …
Experimental and theoretical study on the microparticle trap** and release in a deformable nano-sieve channel
Deformable microfluidics may be potentially used in cell manipulation, optical sensing, and
imaging applications, and have drawn considerable scientific interests in the recent past …
imaging applications, and have drawn considerable scientific interests in the recent past …
Technologies for the directed evolution of cell therapies
D Di Carlo - SLAS TECHNOLOGY: Translating Life …, 2019 - journals.sagepub.com
The next generation of therapies is moving beyond the use of small molecules and proteins
to using whole cells. Compared with the interactions of small-molecule drugs with …
to using whole cells. Compared with the interactions of small-molecule drugs with …
Facile Production of Large‐Area Cell Arrays Using Surface‐Assembled Microdroplets
K Perez‐Toralla, A Olivera‐Torres, MA Rose… - Advanced …, 2020 - Wiley Online Library
Techniques that enable the spatial arrangement of living cells into defined patterns are
broadly applicable to tissue engineering, drug screening, and cell–cell investigations …
broadly applicable to tissue engineering, drug screening, and cell–cell investigations …
[HTML][HTML] Automated sensing and splitting of stem cell colonies on microraft arrays
Human induced pluripotent stem cells (hiPSCs) are widely used for disease modeling,
tissue engineering, and clinical applications. Although the development of new disease …
tissue engineering, and clinical applications. Although the development of new disease …
Integrated Sensing of Camber, Aerodynamic Load, and Vortex Structures over Membrane Wings
J Bohnker, K Breuer - AIAA Journal, 2023 - arc.aiaa.org
Due to the compliant nature of extensible membrane wings, there exists a close relationship
between the membrane wing camber and the aerodynamic load. Additionally, the …
between the membrane wing camber and the aerodynamic load. Additionally, the …
Facile fabrication of micropattern surfaces with controlled wettability on PDMS-modified fiber membranes for cell patterning
C **e, B Sun, R Liu, K Qu, W Zhang, Z Weng… - Analytical …, 2022 - pubs.rsc.org
Various cell culture substrates have been developed for cell patterning to control cell
distributions and orientations in tissue engineering, drug screening and regenerative …
distributions and orientations in tissue engineering, drug screening and regenerative …
Stem/Proliferative and Differentiated Cells within Primary Murine Colonic Epithelium Display Distinct Intracellular Free Ca2+ Signal Codes
The second messenger, intracellular free calcium (Ca2+), acts to transduce mitogenic and
differentiation signals incoming to the colonic epithelium. A self‐renewing monolayer of …
differentiation signals incoming to the colonic epithelium. A self‐renewing monolayer of …