Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors

EC Wheeler, AQ Vu, JM Einstein, M DiSalvo… - Nature …, 2020 - nature.com
Genetic screens using pooled CRISPR-based approaches are scalable and inexpensive,
but restricted to standard readouts, including survival, proliferation and sortable markers …

A technology of a different sort: microraft arrays

B Cortés-Llanos, Y Wang, CE Sims, NL Allbritton - Lab on a Chip, 2021 - pubs.rsc.org
A common procedure performed throughout biomedical research is the selection and
isolation of biological entities such as organelles, cells and organoids from a mixed …

Injection molded microfluidics for establishing high-density single cell arrays in an open hydrogel format

Y Li, JD Motschman, ST Kelly, BB Yellen - Analytical chemistry, 2020 - ACS Publications
Here, we develop an injection molded microfluidic approach for single cell analysis by
making use of (1) rapidly curing injectable hydrogels,(2) a high density microfluidic weir trap …

Experimental and theoretical study on the microparticle trap** and release in a deformable nano-sieve channel

X Chen, L Falzon, J Zhang, X Zhang, RQ Wang… - …, 2019 - iopscience.iop.org
Deformable microfluidics may be potentially used in cell manipulation, optical sensing, and
imaging applications, and have drawn considerable scientific interests in the recent past …

Technologies for the directed evolution of cell therapies

D Di Carlo - SLAS TECHNOLOGY: Translating Life …, 2019 - journals.sagepub.com
The next generation of therapies is moving beyond the use of small molecules and proteins
to using whole cells. Compared with the interactions of small-molecule drugs with …

Facile Production of Large‐Area Cell Arrays Using Surface‐Assembled Microdroplets

K Perez‐Toralla, A Olivera‐Torres, MA Rose… - Advanced …, 2020 - Wiley Online Library
Techniques that enable the spatial arrangement of living cells into defined patterns are
broadly applicable to tissue engineering, drug screening, and cell–cell investigations …

[HTML][HTML] Automated sensing and splitting of stem cell colonies on microraft arrays

M DiSalvo, NM Smiddy, NL Allbritton - APL bioengineering, 2019 - pubs.aip.org
Human induced pluripotent stem cells (hiPSCs) are widely used for disease modeling,
tissue engineering, and clinical applications. Although the development of new disease …

Integrated Sensing of Camber, Aerodynamic Load, and Vortex Structures over Membrane Wings

J Bohnker, K Breuer - AIAA Journal, 2023 - arc.aiaa.org
Due to the compliant nature of extensible membrane wings, there exists a close relationship
between the membrane wing camber and the aerodynamic load. Additionally, the …

Facile fabrication of micropattern surfaces with controlled wettability on PDMS-modified fiber membranes for cell patterning

C **e, B Sun, R Liu, K Qu, W Zhang, Z Weng… - Analytical …, 2022 - pubs.rsc.org
Various cell culture substrates have been developed for cell patterning to control cell
distributions and orientations in tissue engineering, drug screening and regenerative …

Stem/Proliferative and Differentiated Cells within Primary Murine Colonic Epithelium Display Distinct Intracellular Free Ca2+ Signal Codes

S Mestril, R Kim, SS Hinman… - Advanced …, 2021 - Wiley Online Library
The second messenger, intracellular free calcium (Ca2+), acts to transduce mitogenic and
differentiation signals incoming to the colonic epithelium. A self‐renewing monolayer of …