The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

The spontaneous deamination of cytosine is a major source of transitions from C• G to T• A
base pairs, which account for half of known pathogenic point mutations in humans. The …

RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …

Base editors have substantial promise in basic research and as therapeutic agents for the
correction of pathogenic mutations. The development of adenine transversion editors has …

Base excision repair (BER) corrects DNA damage from oxidation, deamination and
alkylation. Such base lesions cause little distortion to the DNA helix structure. BER is …

Although base editors are widely used to install targeted point mutations, the factors that
determine base editing outcomes are not well understood. We characterized sequence …

Chromothripsis is a catastrophic mutational process that promotes tumorigenesis and
causes congenital disease,,–. Chromothripsis originates from aberrations of nuclei called …

Mutagenesis of protein-encoding sequences occurs ubiquitously; it enables evolution,
accumulates during aging, and is associated with disease. Many biotechnological methods …

Alkylation lesions in DNA and RNA result from endogenous compounds, environmental
agents and alkylating drugs. Simple methylating agents, eg methylnitrosourea, tobacco …