Bottom-up proteomics: advancements in sample preparation

VA Duong, H Lee - International journal of molecular sciences, 2023 - mdpi.com
Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a
powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom …

Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications

XR Liu, MM Zhang, ML Gross - Chemical reviews, 2020 - ACS Publications
Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Protein analysis by shotgun/bottom-up proteomics

Y Zhang, BR Fonslow, B Shan, MC Baek… - Chemical …, 2013 - ACS Publications
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

The human epididymis: its function in sperm maturation

R Sullivan, R Mieusset - Human reproduction update, 2016 - academic.oup.com
BACKGROUND Spermatozoa acquire their fertilizing ability and forward motility properties
during epididymal transit. Our knowledge of gamete physiology is based on studies …

Stable isotope labelling methods in mass spectrometry-based quantitative proteomics

O Chahrour, D Cobice, J Malone - Journal of pharmaceutical and …, 2015 - Elsevier
Mass-spectrometry based proteomics has evolved as a promising technology over the last
decade and is undergoing a dramatic development in a number of different areas, such as; …

Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing

P Galanos, K Vougas, D Walter, A Polyzos… - Nature cell …, 2016 - nature.com
The cyclin-dependent kinase inhibitor p21WAF1/CIP1 (p21) is a cell-cycle checkpoint
effector and inducer of senescence, regulated by p53. Yet, evidence suggests that p21 could …

Proteomic techniques for characterisation of mesenchymal stem cell secretome

HK Skalnikova - Biochimie, 2013 - Elsevier
Mesenchymal stem cells (MSCs) are multipotent cells with a substantial potential in human
regenerative medicine due to their ability to migrate to sites of injury, capability to suppress …

Review of three-dimensional liquid chromatography platforms for bottom-up proteomics

VA Duong, JM Park, H Lee - International Journal of Molecular Sciences, 2020 - mdpi.com
Proteomics is a large-scale study of proteins, aiming at the description and characterization
of all expressed proteins in biological systems. The expressed proteins are typically highly …

Recent developments in high-performance liquid chromatography stationary phases

TL Chester - Analytical chemistry, 2013 - ACS Publications
Experts and suppliers continue to make advances in the application of theory, capabilities,
and practice of highperformance liquid chromatography (HPLC). It is one of the most often …

Proteomics in forensic analysis: Applications for human samples

VA Duong, JM Park, HJ Lim, H Lee - Applied Sciences, 2021 - mdpi.com
Proteomics, the large-scale study of all proteins of an organism or system, is a powerful tool
for studying biological systems. It can provide a holistic view of the physiological and …