Liposome-chaperoned cell-free synthesis for the design of proteoliposomes: Implications for therapeutic delivery

M Lu, X Zhao, H **ng, Z Xun, T Yang, C Cai, D Wang… - Acta Biomaterialia, 2018 - Elsevier
Cell-free (CF) protein synthesis has emerged as a powerful technique platform for efficient
protein production in vitro. Liposomes have been widely studied as therapeutic carriers due …

Compartmentalized reactions as a case of soft-matter biotechnology: synthesis of proteins and nucleic acids inside lipid vesicles

P Stano, P Carrara, Y Kuruma, TP de Souza… - Journal of Materials …, 2011 - pubs.rsc.org
In this mini-review we would like to summarize the recent advances in the field of protein
and nucleic acid synthesis inside lipid vesicles (liposomes). This research, which originated …

Minimal cells: Relevance and interplay of physical and biochemical factors

P Stano - Biotechnology Journal, 2011 - Wiley Online Library
Research on the construction of minimal cell‐like systems is continuously progressing. The
aim is to assemble a synthetic or semi‐synthetic cell by encapsulating the minimal set of …

Pyrrhocoricin, a proline-rich antimicrobial peptide derived from insect, inhibits the translation process in the cell-free Escherichia coli protein synthesis system

M Taniguchi, A Ochiai, H Kondo, S Fukuda… - Journal of bioscience …, 2016 - Elsevier
Previous studies have shown that pyrrhocoricin, a proline-rich antimicrobial peptide
(PrAMP), killed sensitive species in a dose-dependent manner by specifically binding to …

Cell-free protein synthesis of influenza virus hemagglutinin HA2-integrated virosomes for siRNA delivery

Y Wang, B **, B Li, Y Luo, M Ma, Y Chen, H Liu… - International Journal of …, 2022 - Elsevier
It is well known that the difficulty of siRNA therapeutic application is the lack of safe and
effective delivery vector. Virosome is a nano vesicle composed of lipid membrane and …

Enhanced adsorption performance of varying-length mRNA on Oligo dT affinity resins through optimal pore size and grafting

YZ Tan, LZ Qiao, SS Wang, J Zhang, J Qian… - Biochemical …, 2024 - Elsevier
The enormous potential of mRNA-based therapeutics in vaccines and medicine has led to
the consideration of various mRNA sizes, ranging from∼ 1000 to 10,000 nucleotides (nt) …

Conformational change of single-stranded RNAs induced by liposome binding

K Suga, T Tanabe, H Tomita… - Nucleic acids …, 2011 - academic.oup.com
The interaction between single-stranded RNAs and liposomes was studied using UV,
Fourier Transform Infrared spectroscopy (FTIR) and Circular Dichroism spectroscopy (CD) …

Heterogeneous Cationic Liposomes Modified with 3β-{N-[(N′,N′-Dimethylamino)ethyl]carbamoyl}cholesterol Can Induce Partial Conformational Changes in …

K Suga, T Tanabe, H Umakoshi - Langmuir, 2013 - ACS Publications
The effect of cationic liposomes (CLs) on messenger RNA (mRNA) conformation and
translation was studied, focusing on membrane heterogeneity. CLs, composed of 1, 2 …

Inorganic nanovectors for nucleic acid delivery

S Pranatharthiharan, MD Patel, AA D'Souza… - Drug delivery and …, 2013 - Springer
Nucleic acids show immense potential to treat cancer, acquired immune deficiency
syndrome, neurological diseases and other incurable human diseases. Upon systemic …

AmyI-1–18, a cationic α-helical antimicrobial octadecapeptide derived from α-amylase in rice, inhibits the translation and folding processes in a protein synthesis …

M Taniguchi, A Ochiai, S Fukuda, T Sato… - Journal of bioscience …, 2016 - Elsevier
In our previous study, we used a cell-free rapid translation system (RTS), which is an in vitro
protein synthesis system based on Escherichia coli lysate, for evaluating the inhibition of …