Highlights•A review of flow cytometry for monitoring probiotic culture viability was
undertaken.•Flow cytometry had good correlation with plate counts for concentrated fresh …

Flow cytometry (FC) is a powerful technique for the rapid analysis of single cells in a mixture,
by means of light-scattering and fluorescence measurements. In biotechnology, the power of …

The human gut contains trillions of microorganisms that influence our health by metabolizing
xenobiotics, including host-targeted drugs and antibiotics. Recent efforts have characterized …

As bacteria transition from exponential to stationary phase, they change substantially in size,
morphology, growth and expression profiles. These responses also vary between individual …

Flow cytometric monitoring of propidium iodide (PI) uptake is a well‐established and rapid
method for monitoring cell death and is used on the basis that the intact membrane of viable …

ABSTRACT A novel approach combining a flow cytometric in situ viability assay with 16S
rRNA gene analysis was used to study the relationship between diversity and activity of the …

Optical tweezers offer a non-contact method for selecting single cells and translocating them
from one microenvironment to another. We have characterized the optical tweezing of yeast …

Population heterogeneity is omnipresent in all bioprocesses even in homogenous
environments. Its origin, however, is only so well understood that potential strategies like bet …

With the continuous development, in the last decades, of analytical techniques providing
complex information at single cell level, the study of cell heterogeneity has been the focus of …

The field of microbiology has in recent years been transformed by the ever increasing
number of publicly available whole-genome sequences. This sequence information has …