Hot spots-making directed evolution easier

H Yu, S Ma, Y Li, PA Dalby - Biotechnology Advances, 2022 - Elsevier
Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

[HTML][HTML] Tunnel engineering of gas-converting enzymes for inhibitor retardation and substrate acceleration

SM Kim, SH Kang, BW Jeon, YH Kim - Bioresource Technology, 2024 - Elsevier
Carbon monoxide dehydrogenase (CODH), formate dehydrogenase (FDH), hydrogenase
(H2ase), and nitrogenase (N2ase) are crucial enzymatic catalysts that facilitate the …

Graphene-metamaterial based tunable broadband polarization insensitive absorber for terahertz antenna design

R Rahad, ASM Mohsin, B Bhuian, MM Rahman - Ieee Access, 2024 - ieeexplore.ieee.org
The advent of graphene has opened new avenues for the design of high-performance
metamaterial devices, particularly in the realm of electromagnetic absorption applications …

One-pot chemobiocatalytic production of 2, 5-bis (hydroxymethyl) furan and its diester from biomass in aqueous media

Q Wu, MH Zong, N Li - ACS Catalysis, 2023 - ACS Publications
Direct valorization of biomass into value-added chemicals is attractive yet challenging.
Herein, we report a one-pot synthesis of 2, 5-bis (hydroxymethyl) furan (BHMF) and its …

An Engineered Imine Reductase for Highly Diastereo‐and Enantioselective Synthesis of β‐Branched Amines with Contiguous Stereocenters

ZY Zhu, M Shi, CL Li, YF Gao, XY Shen… - Angewandte …, 2024 - Wiley Online Library
Abstract β‐Branched chiral amines with contiguous stereocenters are valuable building
blocks for preparing various biologically active molecules. However, their asymmetric …

In-depth analysis of biocatalysts by microfluidics: An emerging source of data for machine learning

M Vasina, D Kovar, J Damborsky, Y Ding, T Yang… - Biotechnology …, 2023 - Elsevier
Nowadays, the vastly increasing demand for novel biotechnological products is supported
by the continuous development of biocatalytic applications that provide sustainable green …

Biocatalytic gateway to convert glycerol into 3-hydroxypropionic acid in waste-based biorefineries: Fundamentals, limitations, and potential research strategies

HM Zabed, S Akter, PF Rupani, J Akor, Y Zhang… - Biotechnology …, 2023 - Elsevier
Microbial conversion of bioenergy-derived waste glycerol into value-added chemicals has
emerged as an important bioprocessing technology due to its eco-friendliness, feasible …

Frustration and the kinetic repartitioning mechanism of substrate inhibition in enzyme catalysis

Y Zhang, M Chen, J Lu, W Li… - The Journal of …, 2022 - ACS Publications
Substrate inhibition, whereby enzymatic activity decreases with excess substrate after
reaching a maximum turnover rate, is among the most elusive phenomena in enzymatic …

[HTML][HTML] Partial reversible inhibition of enzymes and its metabolic and pharmaco-toxicological implications

P Masson, AR Mukhametgalieva - International Journal of Molecular …, 2023 - mdpi.com
Partial reversible inhibition of enzymes, also called hyperbolic inhibition, is an uncommon
mechanism of reversible inhibition, resulting from a productive enzyme–inhibitor complex …

Characterization of the Ensemble of Lignin-Remodeling DyP-Type Peroxidases from Streptomyces coelicolor A3(2)

H Pupart, P Jõul, MI Bramanis, T Lukk - Energies, 2023 - mdpi.com
Lignin is Nature's major source of aromatic chemistry and is by many seen as the green
entry-point alternative to the fossil-based chemical industry. Due to its chemically recalcitrant …