A field guide to whole‐genome sequencing, assembly and annotation

R Ekblom, JBW Wolf - Evolutionary applications, 2014 - Wiley Online Library
Genome sequencing projects were long confined to biomedical model organisms and
required the concerted effort of large consortia. Rapid progress in high‐throughput …

Technology dictates algorithms: recent developments in read alignment

M Alser, J Rotman, D Deshpande, K Taraszka, H Shi… - Genome biology, 2021 - Springer
Aligning sequencing reads onto a reference is an essential step of the majority of genomic
analysis pipelines. Computational algorithms for read alignment have evolved in …

Assembly of allele-aware, chromosomal-scale autopolyploid genomes based on Hi-C data

X Zhang, S Zhang, Q Zhao, R Ming, H Tang - Nature plants, 2019 - nature.com
Construction of chromosome-level assembly is a vital step in achieving the goal of a
'Platinum'genome, but it remains a major challenge to assemble and anchor sequences to …

Genomes of 13 domesticated and wild rice relatives highlight genetic conservation, turnover and innovation across the genus Oryza

JC Stein, Y Yu, D Copetti, DJ Zwickl, L Zhang… - Nature …, 2018 - nature.com
The genus Oryza is a model system for the study of molecular evolution over time scales
ranging from a few thousand to 15 million years. Using 13 reference genomes spanning the …

[HTML][HTML] 1,135 genomes reveal the global pattern of polymorphism in Arabidopsis thaliana

C Alonso-Blanco, J Andrade, C Becker, F Bemm… - Cell, 2016 - cell.com
Arabidopsis thaliana serves as a model organism for the study of fundamental physiological,
cellular, and molecular processes. It has also greatly advanced our understanding of …

The pangenome of an agronomically important crop plant Brassica oleracea

AA Golicz, PE Bayer, GC Barker, PP Edger… - Nature …, 2016 - nature.com
There is an increasing awareness that as a result of structural variation, a reference
sequence representing a genome of a single individual is unable to capture all of the gene …

Origin and evolution of the triploid cultivated banana genome

X Li, S Yu, Z Cheng, X Chang, Y Yun, M Jiang… - Nature …, 2024 - nature.com
Most fresh bananas belong to the Cavendish and Gros Michel subgroups. Here, we report
chromosome-scale genome assemblies of Cavendish (1.48 Gb) and Gros Michel (1.33 Gb) …

A map of rice genome variation reveals the origin of cultivated rice

X Huang, N Kurata, X Wei, ZX Wang, A Wang, Q Zhao… - Nature, 2012 - nature.com
Crop domestications are long-term selection experiments that have greatly advanced
human civilization. The domestication of cultivated rice (Oryza sativa L.) ranks as one of the …

Chromosome-level assemblies of multiple Arabidopsis genomes reveal hotspots of rearrangements with altered evolutionary dynamics

WB Jiao, K Schneeberger - Nature communications, 2020 - nature.com
Despite hundreds of sequenced Arabidopsis genomes, very little is known about the degree
of genomic collinearity within single species, due to the low number of chromosome-level …

High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell

TP Michael, F Jupe, F Bemm, ST Motley… - Nature …, 2018 - nature.com
Abstract The handheld Oxford Nanopore MinION sequencer generates ultra-long reads with
minimal cost and time requirements, which makes sequencing genomes at the bench …