Force spectroscopy of single cells using atomic force microscopy

A Viljoen, M Mathelié-Guinlet, A Ray… - Nature Reviews …, 2021 - nature.com
Physical forces and mechanical properties have critical roles in cellular function, physiology
and disease. Over the past decade, atomic force microscopy (AFM) techniques have …

Atomic force microscopy-based force spectroscopy and multiparametric imaging of biomolecular and cellular systems

DJ Müller, AC Dumitru, C Lo Giudice… - Chemical …, 2020 - ACS Publications
During the last three decades, a series of key technological improvements turned atomic
force microscopy (AFM) into a nanoscopic laboratory to directly observe and chemically …

Force-distance curves by atomic force microscopy

B Cappella, G Dietler - Surface science reports, 1999 - Elsevier
Atomic force microscopy (AFM) force-distance curves have become a fundamental tool in
several fields of research, such as surface science, materials engineering, biochemistry and …

Covalent mechanochemistry: theoretical concepts and computational tools with applications to molecular nanomechanics

J Ribas-Arino, D Marx - Chemical reviews, 2012 - ACS Publications
In the following section, we will recapitulate selected experimental milestones through the
eyes of the theoretician, which, in our opinion, significantly advanced the field of covalent …

Detection and localization of single molecular recognition events using atomic force microscopy

P Hinterdorfer, YF Dufrêne - Nature methods, 2006 - nature.com
Because of its piconewton force sensitivity and nanometer positional accuracy, the atomic
force microscope (AFM) has emerged as a powerful tool for exploring the forces and the …

Dynamic force spectroscopy of single DNA molecules

T Strunz, K Oroszlan, R Schäfer… - Proceedings of the …, 1999 - pnas.org
To explore the analytic relevance of unbinding force measurements between
complementary DNA strands with an atomic force microscope, we measured the forces to …

Force probing surfaces of living cells to molecular resolution

DJ Müller, J Helenius, D Alsteens… - Nature chemical biology, 2009 - nature.com
Biological processes rely on molecular interactions that can be directly measured using
force spectroscopy techniques. Here we review how atomic force microscopy can be applied …

Single-molecule experiments in biological physics: methods and applications

F Ritort - Journal of Physics: Condensed Matter, 2006 - iopscience.iop.org
I review single-molecule experiments (SMEs) in biological physics. Recent technological
developments have provided the tools to design and build scientific instruments of high …

Unfolding pathways of individual bacteriorhodopsins

F Oesterhelt, D Oesterhelt, M Pfeiffer, A Engel… - Science, 2000 - science.org
Atomic force microscopy and single-molecule force spectroscopy were combined to image
and manipulate purple membrane patches from Halobacterium salinarum. Individual …

Proteoglycans of the extracellular environment: clues from the gene and protein side offer novel perspectives in molecular diversity and function

RV Iozzo, AD Murdoch - The FASEB Journal, 1996 - Wiley Online Library
This review focuses on the extracellular proteoglycans. Special emphasis is placed on the
structural features of their protein cores, their gene organization, and their transcriptional …