Next-generation physiology approaches to study microbiome function at single cell level

R Hatzenpichler, V Krukenberg, RL Spietz… - Nature Reviews …, 2020 - nature.com
The function of cells in their native habitat often cannot be reliably predicted from genomic
data or from physiology studies of isolates. Traditional experimental approaches to study the …

[HTML][HTML] Optical tweezers: Phototoxicity and thermal stress in cells and biomolecules

A Blázquez-Castro - Micromachines, 2019 - mdpi.com
For several decades optical tweezers have proven to be an invaluable tool in the study and
analysis of myriad biological responses and applications. However, as with every tool, they …

[HTML][HTML] Dissecting horizontal and vertical gene transfer of antibiotic resistance plasmid in bacterial community using microfluidics

B Li, Y Qiu, Y Song, H Lin, H Yin - Environment International, 2019 - Elsevier
The spread of antibiotic resistance genes (ARGs) has become an emerging threat to the
global health. Although horizontal gene transfer (HGT) is regarded as one of the major …

Raman spectroscopy combined with machine learning for rapid detection of food-borne pathogens at the single-cell level

S Yan, S Wang, J Qiu, M Li, D Li, D Xu, D Li, Q Liu - Talanta, 2021 - Elsevier
Rapid detection of food-borne pathogens in early food contamination is a permanent topic to
ensure food safety and prevent public health problems. Raman spectroscopy, a label-free …

A single-cell Raman-based platform to identify developmental stages of human pluripotent stem cell-derived neurons

CC Hsu, J Xu, B Brinkhof, H Wang, Z Cui… - Proceedings of the …, 2020 - pnas.org
Stem cells with the capability to self-renew and differentiate into multiple cell derivatives
provide platforms for drug screening and promising treatment options for a wide variety of …

Ramanome technology platform for label-free screening and sorting of microbial cell factories at single-cell resolution

Y He, X Wang, B Ma, J Xu - Biotechnology Advances, 2019 - Elsevier
Phenotypic profiling of natural, engineered or synthetic cells has increasingly become a
bottleneck in the mining and engineering of cell factories. Single-cell phenoty** …

High-speed diagnosis of bacterial pathogens at the single cell level by Raman microspectroscopy with machine learning filters and denoising autoencoders

J Xu, X Yi, G **, D Peng, G Fan, X Xu… - ACS Chemical …, 2022 - ACS Publications
Accurate and rapid identification of infectious bacteria is important in medicine. Raman
microspectroscopy holds great promise in performing label-free identification at the single …

Optofluidic Raman-activated cell sorting for targeted genome retrieval or cultivation of microbial cells with specific functions

KS Lee, FC Pereira, M Palatinszky, L Behrendt… - Nature …, 2021 - nature.com
Stable isotope labeling of microbial taxa of interest and their sorting provide an efficient and
direct way to answer the question “who does what?” in complex microbial communities …

High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells

Y Ma, Y Lee, C Best-Popescu, L Gao - Proceedings of the National …, 2021 - pnas.org
We present high-resolution, high-speed fluorescence lifetime imaging microscopy (FLIM) of
live cells based on a compressed sensing scheme. By leveraging the compressibility of …

Self-assembled plasmonic nanoarrays for enhanced bacterial identification and discrimination

T Tian, J Yi, Y Liu, B Li, Y Liu, L Qiao, K Zhang… - Biosensors and …, 2022 - Elsevier
The rapid and accurate bacterial testing is a critical step for the management of infectious
diseases, but challenges remain largely due to a lack of advanced sensing tools. Here we …