Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical
technique being increasingly applied to a wide variety of problems. As the HDX-MS …

Hydrogen/deuterium exchange (HDX) mass spectrometry (MS) has become a key technique
for monitoring structural and dynamic aspects of proteins in solution. This approach relies on …

Highly dynamic mitotic spindle microtubules are superb therapeutic targets for a group of
chemically diverse and clinically successful anticancer drugs. Microtubule-targeted drugs …

Polymerase theta (Polθ) acts in DNA replication and repair, and its inhibition is synthetic
lethal in BRCA1 and BRCA2-deficient tumor cells. Novobiocin (NVB) is a first-in-class …

Functional regulation of ligand-activated receptors is driven by alterations in the
conformational dynamics of the protein upon ligand binding. Differential hydrogen/deuterium …

Highlights•Hydrogen/deuterium exchange mass spectrometry (HDX-MS) technology for
assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS …

The microtubule (MT) associated protein tau, which is highly expressed in the axons of
neurons, is an endogenous MT-stabilizing agent that plays an important role in axonal …

Laulimalide and peloruside A are microtubule‐stabilizing agents (MSAs), the mechanism of
action on microtubules of which is poorly defined. Here, using X‐ray crystallography it is …

Differential hydrogen exchange-mass spectrometry (HX-MS) measurements are valuable for
identification of differences in the higher order structures of proteins. Typically, the data sets …

Assessing the functional outcome of protein interactions in structural terms is a goal of
structural biology, however most techniques have a limited capacity for making structure …