Bridging live-cell imaging and next-generation cancer treatment

M Alieva, AKL Wezenaar, EJ Wehrens… - Nature Reviews Cancer, 2023‏ - nature.com
By providing spatial, molecular and morphological data over time, live-cell imaging can
provide a deeper understanding of the cellular and signalling events that determine cancer …

Deep 3D histology powered by tissue clearing, omics and AI

A Ertürk - Nature methods, 2024‏ - nature.com
To comprehensively understand tissue and organism physiology and pathophysiology, it is
essential to create complete three-dimensional (3D) cellular maps. These maps require …

CellSighter: a neural network to classify cells in highly multiplexed images

Y Amitay, Y Bussi, B Feinstein, S Bagon, I Milo… - Nature …, 2023‏ - nature.com
Multiplexed imaging enables measurement of multiple proteins in situ, offering an
unprecedented opportunity to chart various cell types and states in tissues. However, cell …

Recent advances in carbon dots-based nanoplatforms: physicochemical properties and biomedical applications

S Rui, L Song, J Lan, D Wang, S Feng, J Lu… - Chemical Engineering …, 2023‏ - Elsevier
Carbon dots (CDs) are a new type of fluorescent carbon nanomaterials with highly
carbonized cores and surface groups. The outstanding properties of CDs, such as excellent …

[HTML][HTML] Two-photon synthetic aperture microscopy for minimally invasive fast 3D imaging of native subcellular behaviors in deep tissue

Z Zhao, Y Zhou, B Liu, J He, J Zhao, Y Cai, J Fan, X Li… - Cell, 2023‏ - cell.com
Holistic understanding of physio-pathological processes requires noninvasive 3D imaging in
deep tissue across multiple spatial and temporal scales to link diverse transient subcellular …

Multiphoton fluorescence microscopy for in vivo imaging

C Xu, M Nedergaard, DJ Fowell, P Friedl, N Ji - Cell, 2024‏ - cell.com
Multiphoton fluorescence microscopy (MPFM) has been a game-changer for optical
imaging, particularly for studying biological tissues deep within living organisms. MPFM …

Deep self-learning enables fast, high-fidelity isotropic resolution restoration for volumetric fluorescence microscopy

K Ning, B Lu, X Wang, X Zhang, S Nie, T Jiang… - Light: Science & …, 2023‏ - nature.com
One intrinsic yet critical issue that troubles the field of fluorescence microscopy ever since its
introduction is the unmatched resolution in the lateral and axial directions (ie, resolution …

Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopy

C Qiao, Y Zeng, Q Meng, X Chen, H Chen… - Nature …, 2024‏ - nature.com
Computational super-resolution methods, including conventional analytical algorithms and
deep learning models, have substantially improved optical microscopy. Among them …

Multi-focus light-field microscopy for high-speed large-volume imaging

Y Zhang, Y Wang, M Wang, Y Guo, X Li, Y Chen, Z Lu… - PhotoniX, 2022‏ - Springer
High-speed visualization of three-dimensional (3D) processes across a large field of view
with cellular resolution is essential for understanding living systems. Light-field microscopy …

Self-supervised denoising for multimodal structured illumination microscopy enables long-term super-resolution live-cell imaging

X Chen, C Qiao, T Jiang, J Liu, Q Meng, Y Zeng… - PhotoniX, 2024‏ - Springer
Detection noise significantly degrades the quality of structured illumination microscopy (SIM)
images, especially under low-light conditions. Although supervised learning based …