Optical tweezers in single-molecule biophysics
Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …
studies. In this Primer, we first review the physical principles of optical tweezers and the …
FRET as a biomolecular research tool—understanding its potential while avoiding pitfalls
The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …
However, different FRET techniques are not applied consistently, nor are results uniformly …
Extreme dynamics in a biomolecular condensate
Proteins and nucleic acids can phase-separate in the cell to form concentrated biomolecular
condensates,,–. The functions of condensates span many length scales: they modulate …
condensates,,–. The functions of condensates span many length scales: they modulate …
Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins
Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study
of biomolecular structure and dynamics in vitro and in vivo. We performed an international …
of biomolecular structure and dynamics in vitro and in vivo. We performed an international …
[HTML][HTML] Pursuing excitonic energy transfer with programmable DNA-based optical breadboards
DNA nanotechnology has now enabled the self-assembly of almost any prescribed 3-
dimensional nanoscale structure in large numbers and with high fidelity. These structures …
dimensional nanoscale structure in large numbers and with high fidelity. These structures …
Single-molecule FRET imaging of GPCR dimers in living cells
WB Asher, P Geggier, MD Holsey, GT Gilmore… - Nature …, 2021 - nature.com
Class CG protein-coupled receptors (GPCRs) are known to form stable homodimers or
heterodimers critical for function, but the oligomeric status of class A and B receptors, which …
heterodimers critical for function, but the oligomeric status of class A and B receptors, which …
Visualizing the disordered nuclear transport machinery in situ
The approximately 120 MDa mammalian nuclear pore complex (NPC) acts as a gatekeeper
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …
Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
Interaction dynamics of intrinsically disordered proteins from single-molecule spectroscopy
Many proteins contain large structurally disordered regions or are entirely disordered under
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …
A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories
Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function
of biomolecules since it makes nanoscale movements detectable as fluorescence signals …
of biomolecules since it makes nanoscale movements detectable as fluorescence signals …