Functional metagenomics as a tool for identification of new antibiotic resistance genes from natural environments

DFK Dos Santos, P Istvan, BF Quirino, RH Kruger - Microbial ecology, 2017 - Springer
Antibiotic resistance has become a major concern for human and animal health, as
therapeutic alternatives to treat multidrug-resistant microorganisms are rapidly dwindling …

ResFinderFG v2. 0: a database of antibiotic resistance genes obtained by functional metagenomics

R Gschwind, S Ugarcina Perovic, M Weiss… - Nucleic Acids …, 2023 - academic.oup.com
Metagenomics can be used to monitor the spread of antibiotic resistance genes (ARGs).
ARGs found in databases such as ResFinder and CARD primarily originate from culturable …

[HTML][HTML] Early genetic diagnosis of clarithromycin resistance in Helicobacter pylori

XH Li, YY Huang, LM Lu, LJ Zhao, XK Luo… - World Journal of …, 2021 - ncbi.nlm.nih.gov
BACKGROUND The drug resistance rate of clinical Helicobacter pylori (H. pylori) isolates
has increased. However, the mechanism of drug resistance remains unclear. In this study …

[HTML][HTML] The Prevalence and the Underlying Mechanisms of Fosfomycin Resistance of Escherichia coli and Salmonella spp. Among Cattle in Japan

Y Hosoi, M Kawanishi, S Harada, M Kumakawa… - International Journal of …, 2024 - mdpi.com
To investigate fosfomycin resistance rates in cattle across Japan, we carried out
susceptibility tests. To identify the genes contributing to fosfomycin resistance, we performed …

Novel soil-derived beta-lactam, chloramphenicol, fosfomycin and trimethoprim resistance genes revealed by functional metagenomics

IM Willms, M Grote, M Kocatürk, L Singhoff, AA Kraft… - Antibiotics, 2021 - mdpi.com
Antibiotic resistance genes (ARGs) in soil are considered to represent one of the largest
environmental resistomes on our planet. As these genes can potentially be disseminated …

Novel biological resources screened from uncultured bacteria by a metagenomic method

N Kimura - Metagenomics, 2018 - Elsevier
Microbial genome analysis has revealed numerous gene sequences, with the potential to
code the enzymes for using in an industrial, agricultural and pharmaceutical application …

Discovery of a new polyhydroxyalkanoate synthase from limestone soil through metagenomic approach

YT Tai, CP Foong, N Najimudin, K Sudesh - Journal of bioscience and …, 2016 - Elsevier
PHA synthase (PhaC) is the key enzyme in the production of biodegradable plastics known
as polyhydroxyalkanoate (PHA). Nevertheless, most of these enzymes are isolated from …

QM/MM study of the fosfomycin resistance mechanism involving FosB enzyme

AH Lima, JRA Silva, CN Alves, J Lameira - ACS omega, 2021 - ACS Publications
Multidrug-resistant organisms contain antibiotic-modifying enzymes that facilitate resistance
to a variety of antimicrobial compounds. Particularly, the fosfomycin (FOF) drug can be …

Computed insight into a peptide inhibitor preventing the induced fit mechanism of MurA enzyme from Pseudomonas aeruginosa

AH Lima, AM Dos Santos, CN Alves… - Chemical Biology & …, 2017 - Wiley Online Library
UDP‐N‐acetylglucosamine enolpyruvyl transferase (MurA) is one of the key enzymes
involved in peptidoglycan biosynthesis. The peptide HESFWYLPHQSY (called PEP 1354) is …

Whole Genome Sequencing of Levilactobacillus brevis HQ1-1 for Understanding the Characteristics of Its Antibiotic Resistance Genes

QH Wu, DD Li, SCL Wu, Y San, Q **ng, H Hu… - Microbiology, 2023 - Springer
Lactic acid bacteria (LAB) are the most commonly used microorganisms in fermented foods.
Currently, in the context of antibiotic resistance (AR), the safe use of LAB has attracted more …