Combined genetic and splicing analysis of BRCA1 c.[594-2A> C; 641A> G] highlights the relevance of naturally occurring in-frame transcripts for develo** disease …

M de La Hoya, O Soukarieh… - Human molecular …, 2016 - academic.oup.com
A recent analysis using family history weighting and co-observation classification modeling
indicated that BRCA1 c. 594-2A> C (IVS9-2A> C), previously described to cause exon 10 …

A review of mismatch repair gene transcripts: issues for interpretation of mRNA splicing assays

BA Thompson, A Martins, AB Spurdle - Clinical genetics, 2015 - Wiley Online Library
Many mismatch repair (MMR) gene disease‐causing mutations identified in cancer patients
result in aberrant messenger RNA (mRNA) splicing. However, mRNA assay interpretation …

Intrinsic disorder and phosphorylation in BRCA2 facilitate tight regulation of multiple conserved binding events

M Julien, R Ghouil, A Petitalot, SM Caputo, A Carreira… - Biomolecules, 2021 - mdpi.com
The maintenance of genome integrity in the cell is an essential process for the accurate
transmission of the genetic material. BRCA2 participates in this process at several levels …

HSF2BP interacts with a conserved domain of BRCA2 and is required for mouse spermatogenesis

I Brandsma, K Sato, SE van Rossum-Fikkert… - Cell reports, 2019 - cell.com
The tumor suppressor BRCA2 is essential for homologous recombination (HR), replication
fork stability, and DNA interstrand crosslink repair in vertebrates. We identify HSF2BP, a …

Adding In Silico Assessment of Potential Splice Aberration to the Integrated Evaluation of BRCA Gene Unclassified Variants

MP Vallée, TL Di Sera, DA Nix, AM Paquette… - Human …, 2016 - Wiley Online Library
Clinical mutation screening of the cancer susceptibility genes BRCA1 and BRCA2
generates many unclassified variants (UVs). Most of these UVs are either rare missense …

Naturally occurring BRCA2 alternative mRNA splicing events in clinically relevant samples

JD Fackenthal, T Yoshimatsu, B Zhang… - Journal of medical …, 2016 - jmg.bmj.com
Background BRCA1 and BRCA2 are the two principal tumour suppressor genes associated
with inherited high risk of breast and ovarian cancer. Genetic testing of BRCA1/2 will often …

Functional evaluation of BRCA2 variants map** to the PALB2-binding and C-terminal DNA-binding domains using a mouse ES cell-based assay

K Biswas, R Das, JM Eggington, H Qiao… - Human molecular …, 2012 - academic.oup.com
Single-nucleotide substitutions and small in-frame insertions or deletions identified in
human breast cancer susceptibility genes BRCA1 and BRCA2 are frequently classified as …

Targeted RNA‐seq successfully identifies normal and pathogenic splicing events in breast/ovarian cancer susceptibility and Lynch syndrome genes

RD Brandão, K Mensaert… - … journal of cancer, 2019 - Wiley Online Library
A subset of genetic variants found through screening of patients with hereditary breast and
ovarian cancer syndrome (HBOC) and Lynch syndrome impact RNA splicing. Through target …

Alternative mRNA splicing can attenuate the pathogenicity of presumed loss-of-function variants in BRCA2

RLS Mesman, FMGR Calléja, M de la Hoya… - Genetics in …, 2020 - nature.com
Purpose Current interpretation guidelines for germline variants in high-risk cancer
susceptibility genes consider predicted loss-of-function (LoF) variants, such as nonsense …

Comparative In Vitro and In Silico Analyses of Variants in Splicing Regions of BRCA1 and BRCA2 Genes and Characterization of Novel Pathogenic Mutations

M Colombo, G De Vecchi, L Caleca, C Foglia… - PloS one, 2013 - journals.plos.org
Several unclassified variants (UVs) have been identified in splicing regions of disease-
associated genes and their characterization as pathogenic mutations or benign …