Artificial modified nucleotides for the electrochemical detection of nucleic acid amplification products

EV Suprun, SA Khmeleva, KG Ptitsyn… - Journal of Analytical …, 2024 - Springer
The review describes the fundamental electrochemical properties of nucleic acids
manifested on solid electrodes, with an emphasis on the spatial structure of …

Development of multiplex RT-PCR with immobilized primers for identification of infectious human pneumonia pathogens

SA Lapa, RA Miftakhov, ES Klochikhina, YI Ammur… - Molecular Biology, 2021 - Springer
A prototype of a system for the detection of infectious human pneumonia pathogens based
on multiplex solid-phase reverse transcription PCR (RT-PCR) was developed. Primers were …

Comparison of pre-labelled primers and nucleotides as DNA labelling method for lateral flow detection of Legionella pneumophila amplicons

C Warmt, J Nagaba, J Henkel - Scientific Reports, 2024 - nature.com
Labelling of nucleic acid amplicons during polymerase chain reaction (PCR) or isothermal
techniques is possible by using both labelled primers and labelled nucleotides. While the …

The EIMB hydrogel microarray technology: thirty years later

DA Gryadunov, BL Shaskolskiy… - Acta Naturae …, 2018 - cyberleninka.ru
Biological microarrays (biochips) are analytical tools that can be used to implement complex
integrative genomic and proteomic approaches to the solution of problems of personalized …

Polymerase incorporation of Cy5 modified 2′-deoxyuridine-5′-triphosphate into double-and single-stranded DNA for direct electrochemical detection

EV Suprun, KV Bibik, SA Khmeleva, KG Ptitsyn… - Journal of …, 2024 - Elsevier
Abstract The 2′-deoxyuridine-5′-triphosphate modified with Cy5 cyanine dye (dUTP-Cy5)
was tested as a carrier of electroactive label and as a proper substrate for DNA polymerases …

Effect of poly (dA) stretch in a template sequence on the rate and yield of polymerase chain reaction with electroactive modified 2′-deoxyuridine-5′-triphosphates

SA Khmeleva, EV Suprun, YA Vasileva, KG Ptitsyn… - Microchemical …, 2024 - Elsevier
The effective incorporation of electroactive labeled nucleotides into DNA during its
enzymatic amplification provides the ground for develo** assays based on direct …

Biscarbocyanine dye for fluorescence imaging: Binding with albumin and DNA, cell accumulation, intracellular distribution and molecular modeling

AA Kostyukov, MG Mestergazi, AE Egorov… - Dyes and …, 2023 - Elsevier
The photophysical characteristics of a new bis-carbocyanine dye (BCD) with 4-
methylpyridine fragment in the polymethine chain as well as its interaction with human …

A Modular Approach for the Synthesis of Diverse Heterobifunctional Cyanine Dyes

C Maller, F Schedel, M Köhn - The Journal of Organic Chemistry, 2024 - ACS Publications
Herein, we present a straightforward synthetic route for the design and synthesis of diverse
heterobifunctional cyanine 5 dyes. We optimized the workup by harnessing the pH-and …

Разработка мультиплексной ОТ-ПЦР с иммобилизованными праймерами для идентификации возбудителей инфекционной пневмонии человека

СА Лапа, РА Мифтахов, ЕС Клочихина… - Молекулярная …, 2021 - elibrary.ru
Разработан прототип системы, основанной на мультиплексной ПЦР с обратной
транскрипцией (ОТ-ПЦР) в иммобилизованной фазе, для выявления возбудителей …

Photoactivated biscarbocyanine dye with two conjugated chromophores: complexes with albumin, photochemical and phototoxic properties

AS Radchenko, AA Kostyukov, AA Markova… - Photochemical & …, 2019 - Springer
Complexes of photosensitizers with blood proteins play an essential role in their delivery to
the cell, as well as in the efficacy of photodynamic therapy. Biscarbocyanine dye non …