R-loops as cellular regulators and genomic threats

MP Crossley, M Bocek, KA Cimprich - Molecular cell, 2019 - cell.com
During transcription, the nascent RNA strand can base pair with its template DNA, displacing
the non-template strand as ssDNA and forming a structure called an R-loop. R-loops are …

[HTML][HTML] R loops: from physiological to pathological roles

T García-Muse, A Aguilera - Cell, 2019 - cell.com
DNA-RNA hybrids play a physiological role in cellular processes, but often, they represent
non-scheduled co-transcriptional structures with a negative impact on transcription …

R-ChIP using inactive RNase H reveals dynamic coupling of R-loops with transcriptional pausing at gene promoters

L Chen, JY Chen, X Zhang, Y Gu, R ** via S9. 6-based DNA–RNA immunoprecipitation and high-throughput sequencing
LA Sanz, F Chédin - Nature protocols, 2019 - nature.com
R-loops are prevalent three-stranded non-B DNA structures composed of an RNA–DNA
hybrid and a single strand of DNA. R-loops are implicated in various basic nuclear …

SF3B1 mutation and ATM deletion codrive leukemogenesis via centromeric R-loop dysregulation

M Cusan, H Shen, B Zhang, A Liao… - The Journal of …, 2023 - Am Soc Clin Investig
RNA splicing factor SF3B1 is recurrently mutated in various cancers, particularly in
hematologic malignancies. We previously reported that coexpression of Sf3b1 mutation and …

Thermostability, tunability, and tenacity of RNA as rubbery anionic polymeric materials in nanotechnology and nanomedicine—specific cancer targeting with …

DW Binzel, X Li, N Burns, E Khan, WJ Lee… - Chemical …, 2021 - ACS Publications
RNA nanotechnology is the bottom-up self-assembly of nanometer-scale architectures,
resembling LEGOs, composed mainly of RNA. The ideal building material should be (1) …

[HTML][HTML] HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia

H Luo, G Zhu, MA Eshelman, TK Fung, Q Lai, F Wang… - Molecular cell, 2022 - cell.com
HOTTIP lncRNA is highly expressed in acute myeloid leukemia (AML) driven by MLL
rearrangements or NPM1 mutations to mediate HOXA topologically associated domain …

TDP-43 mutations link Amyotrophic Lateral Sclerosis with R-loop homeostasis and R loop-mediated DNA damage

M Giannini, A Bayona-Feliu, D Sproviero… - PLoS …, 2020 - journals.plos.org
TDP-43 is a DNA and RNA binding protein involved in RNA processing and with structural
resemblance to heterogeneous ribonucleoproteins (hnRNPs), whose depletion sensitizes …

[HTML][HTML] R-loops: formation, function, and relevance to cell stress

DF Allison, GG Wang - Cell stress, 2019 - ncbi.nlm.nih.gov
Exposure of genomic, single-stranded DNA (ssDNA) during transcription and replication
creates opportunities for the formation of inappropriate secondary structures. Cells manage …

Genomic profiling of native R loops with a DNA-RNA hybrid recognition sensor

K Wang, H Wang, C Li, Z Yin, R **ao, Q Li, Y **ang… - Science …, 2021 - science.org
An R loop is a unique triple-stranded structure that participates in multiple key biological
processes and is relevant to human diseases. Accurate and comprehensive R loop profiling …