Context-specific regulation and function of mRNA alternative polyadenylation

S Mitschka, C Mayr - Nature Reviews Molecular Cell Biology, 2022‏ - nature.com
Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate
mRNA isoforms with alternative 3′ untranslated regions (UTRs). The expression of …

Uncovering the impacts of alternative splicing on the proteome with current omics techniques

M Reixachs‐Solé, E Eyras - Wiley Interdisciplinary Reviews …, 2022‏ - Wiley Online Library
The high‐throughput sequencing of cellular RNAs has underscored a broad effect of isoform
diversification through alternative splicing on the transcriptome. Moreover, the differential …

[HTML][HTML] Sites of transcription initiation drive mRNA isoform selection

C Alfonso-Gonzalez, I Legnini, S Holec, L Arrigoni… - Cell, 2023‏ - cell.com
The generation of distinct messenger RNA isoforms through alternative RNA processing
modulates the expression and function of genes, often in a cell-type-specific manner. Here …

Ultra-accurate microbial amplicon sequencing with synthetic long reads

BJ Callahan, D Grinevich, S Thakur, MA Balamotis… - Microbiome, 2021‏ - Springer
Background Out of the many pathogenic bacterial species that are known, only a fraction are
readily identifiable directly from a complex microbial community using standard next …

Fourth report on chicken genes and chromosomes 2022

J Smith, JM Alfieri, N Anthony, P Arensburger… - … and genome research, 2023‏ - karger.com
The chicken continues to hold its position as a leading model organism within many areas of
research, as well as being a major source of protein for human consumption. The First …

Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons

JD Perez, ST Dieck, B Alvarez-Castelao, G Tushev… - Elife, 2021‏ - elifesciences.org
Although mRNAs are localized in the processes of excitatory neurons, it is still unclear
whether interneurons also localize a large population of mRNAs. In addition, the variability …

Linking transport and translation of mRNAs with endosomes and mitochondria

K Müntjes, SK Devan, AS Reichert, M Feldbrügge - EMBO reports, 2021‏ - embopress.org
In eukaryotic cells, proteins are targeted to their final subcellular locations with precise
timing. A key underlying mechanism is the active transport of cognate mRNAs, which in …

Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoform

H Liu, H Liu, L Wang, L Song, G Jiang, Q Lu… - Nature …, 2023‏ - nature.com
Isoforms of a gene may contribute to diverse biological functions. In the cochlea, the
repertoire of alternative isoforms remains unexplored. We integrated single-cell short-read …

A multi-omics view of neuronal subcellular protein synthesis

C Sun, E Schuman - Current Opinion in Neurobiology, 2023‏ - Elsevier
While it has long been known that protein synthesis is necessary for long-term memory in
the brain, the logistics of neuronal protein synthesis is complicated by the extensive …

Targeted proteoform map** uncovers specific Neurexin-3 variants required for dendritic inhibition

D Hauser, K Behr, K Konno, D Schreiner, A Schmidt… - Neuron, 2022‏ - cell.com
The diversification of cell adhesion molecules by alternative splicing is proposed to underlie
molecular codes for neuronal wiring. Transcriptomic approaches mapped detailed cell-type …