Nanoscale enzymatic compartments in tandem support cascade reactions in vitro
Compartmentalization at the nanoscale is fundamental in nature, where the spatial
segregation of biochemical reactions within cells ensures optimal conditions for regulating …
segregation of biochemical reactions within cells ensures optimal conditions for regulating …
Probing protein stability and proteolytic resistance by loop scanning: a comprehensive mutational analysis
Improvement in protein thermostability was often found to be associated with increase in its
proteolytic resistance as revealed by comparative studies of homologous proteins from …
proteolytic resistance as revealed by comparative studies of homologous proteins from …
'Nunchuck'proteins: Short flexible linkers resist proteolysis by facilitating motions in flanking domains to inhibit the approach of proteases
Peptides linking well-folded and non-interacting domains in fusion proteins can undergo
proteolytic degradation. This leads to physical separation of the domains that were originally …
proteolytic degradation. This leads to physical separation of the domains that were originally …
Directed evolution of biocatalysts
J Sylvestre, H Chautard, F Cedrone… - … process research & …, 2006 - ACS Publications
Biocatalysis is the activity to synthesize chemicals using biological entities, in particular
enzymes. Natural enzymes often need to be optimized before being used in industrial …
enzymes. Natural enzymes often need to be optimized before being used in industrial …
'Super-perfect'enzymes: Structural stabilities and activities of recombinant triose phosphate isomerases from Pyrococcus furiosus and Thermococcus onnurineus …
Triose phosphate isomerases (TIMs) are considered to be 'kinetically perfect'enzymes,
limited in their activity only by the rates of diffusion of substrate and product molecules. Most …
limited in their activity only by the rates of diffusion of substrate and product molecules. Most …
Proteomics of Pyrococcus furiosus, a Hyperthermophilic Archaeon Refractory to Traditional Methods
Pyrococcus furiosus is one of the most extensively studied hyperthermophilic archaea.
Proteins from this hyperthemophile organism are extremely thermostable and are highly …
Proteins from this hyperthemophile organism are extremely thermostable and are highly …
Volsurf computational method applied to the prediction of stability of thermostable enzymes
P Braiuca, A Buthe, C Ebert, P Linda… - Biotechnology Journal …, 2007 - Wiley Online Library
A computational model for the quantitative prediction of protein thermostability has been
developed by means of the Volsurf method. A data set of 22 enzymes of reported …
developed by means of the Volsurf method. A data set of 22 enzymes of reported …
Engineering G protein-coupled receptor expression in bacteria
Approximately 800 genes in hu-mans are dedicated to the pro-duction of a large superfamily
of seven-transmembrane αhelical receptors called G proteincoupled receptors (GPCRs)(1) …
of seven-transmembrane αhelical receptors called G proteincoupled receptors (GPCRs)(1) …
Introduction of a thermophile-sourced ion pair network in the fourth beta/alpha unit of a psychophile-derived triosephosphate isomerase from Methanococcoides …
Hyperthermophile proteins commonly have higher numbers of surface ionic interactions
than homologous proteins from other domains of life. PfuTIM, a triosephosphate isomerase …
than homologous proteins from other domains of life. PfuTIM, a triosephosphate isomerase …
Evidence of native-like substructure (s) in polypeptide chains of carbonic anhydrase deposited into insoluble aggregates during thermal unfolding
A non-specific protease, subtilisin, was used to probe the existence of folded structure in
thermal aggregates of bovine carbonic anhydrase-II (BCA). BCA aggregates and native BCA …
thermal aggregates of bovine carbonic anhydrase-II (BCA). BCA aggregates and native BCA …