Throughout their life, bacteria need to sense and respond to environmental stress. Thus,
such stress responses can require dramatic cellular reprogramming, both at the …

The post-translational hydroxylation of prolyl and lysyl residues, as catalyzed by 2-
oxoglutarate (2OG)-dependent oxygenases, was first identified in collagen biosynthesis …

Translation factor eIF5A, containing the unique amino acid hypusine, was originally shown
to stimulate Met-puromycin synthesis, a model assay for peptide bond formation. More …

Elongation factor P (EF-P) is a translation factor of unknown function that has been
implicated in a great variety of cellular processes. Here, we show that EF-P prevents …

Translation elongation factor P (EF-P) is critical for virulence in bacteria. EF-P is present in
all bacteria and orthologous to archaeal and eukaryotic initiation factor 5A, yet the biological …

Ribosome profiling is a powerful method for globally assessing the activity of ribosomes in a
cell. Despite its application in many organisms, ribosome profiling studies in bacteria have …

Ribosomes synthesizing proteins containing consecutive proline residues become stalled
and require rescue via the action of uniquely modified translation elongation factors, EF-P in …

Efficiency of protein synthesis on the ribosome is strongly affected by the amino acid
composition of the assembled amino acid chain. Challenging sequences include proline …

The prokaryotic translation elongation factor P (EF-P) and the eukaryotic/archaeal
counterparts eIF5A/aIF5A are proteins that serve a crucial role in mitigating ribosomal …

Ribosomes are the protein synthesizing factories of the cell, polymerizing polypeptide
chains from their constituent amino acids. However, distinct combinations of amino acids …