Recombinant protein expression in Escherichia coli: advances and challenges
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …
Its use as a cell factory is well-established and it has become the most popular expression …
Recombinant protein expression in Escherichia coli
F Baneyx - Current opinion in biotechnology, 1999 - Elsevier
Escherichia coli is one of the most widely used hosts for the production of heterologous
proteins and its genetics are far better characterized than those of any other microorganism …
proteins and its genetics are far better characterized than those of any other microorganism …
Recombinant protein folding and misfolding in Escherichia coli
F Baneyx, M Mujacic - Nature biotechnology, 2004 - nature.com
The past 20 years have seen enormous progress in the understanding of the mechanisms
used by the enteric bacterium Escherichia coli to promote protein folding, support protein …
used by the enteric bacterium Escherichia coli to promote protein folding, support protein …
Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements
J Kaur, A Kumar, J Kaur - International journal of biological …, 2018 - Elsevier
E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …
and the availability of improved genetic tools/methods are making it more valuable than …
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli
HP Sørensen, KK Mortensen - Microbial cell factories, 2005 - Springer
Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural
protein sources rarely meet the requirements for quantity, ease of isolation or price and …
protein sources rarely meet the requirements for quantity, ease of isolation or price and …
Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements
Nearly 30% of currently approved recombinant therapeutic proteins are produced in
Escherichia coli. Due to its well-characterized genetics, rapid growth and high-yield …
Escherichia coli. Due to its well-characterized genetics, rapid growth and high-yield …
[PDF][PDF] Protein stability: a crystallographer's perspective
Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food
industries, in addition to being a daily consideration for academic researchers studying …
industries, in addition to being a daily consideration for academic researchers studying …
Production of biopharmaceuticals in E. coli: current scenario and future perspectives
Escherichia coli is the most preferred microorganism to express heterologous proteins for
therapeutic use, as around 30% of the approved therapeutic proteins are currently being …
therapeutic use, as around 30% of the approved therapeutic proteins are currently being …
Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies
Among the various expression systems employed for the over-production of proteins,
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …
Biotechnological uses of enzymes from psychrophiles
The bulk of the Earth's biosphere is cold (eg 90% of the ocean's waters are≤ 5° C),
sustaining a broad diversity of microbial life. The permanently cold environments vary from …
sustaining a broad diversity of microbial life. The permanently cold environments vary from …