Develo** technologies such as unnatural amino acid mutagenesis, non-natural cofactor
engineering, and computational design are generating proteins with novel functions; these …

In this Perspective, we present progress, outstanding challenges, and opportunities for the
incorporation of artificial metalloenzymes (ArMs) into biosynthetic pathways. We first explain …

Enzymes that contain metal ions—that is, metalloenzymes—possess the reactivity of a
transition metal centre and the potential of molecular evolution to modulate the reactivity and …

Tetrapyrrole-containing proteins are one of the most fundamental classes of enzymes in
nature and it remains an open question to give a chemical rationale for the multitude of …

Transition metal catalysts and enzymes possess unique and often complementary
properties that have made them important tools for chemical synthesis. The potential …

The direct conversion of aliphatic Csingle bondH bonds into Csingle bond N bonds provides
an attractive approach to the introduction of nitrogen-containing functionalities in organic …

Hydrogen gas is used extensively in industry today and is often put forward as a suitable
energy carrier due its high energy density. Currently, the main source of molecular hydrogen …

Listeriolysin O (LLO) is a pore-forming toxin that mediates phagosomal escape and cell-to-
cell spread of the intracellular pathogen Listeria monocytogenes. In order to identify factors …

Heme is a cofactor with myriad roles and essential to almost all living organisms. Beyond
classical gas transport and catalytic functions, heme is increasingly appreciated as a tightly …

Conspectus Heme proteins have proven to be a convenient platform for the development of
designer proteins with novel functionalities. This is achieved by substituting the native iron …