[HTML][HTML] Recent advances and perspectives of nucleic acid detection for coronavirus

M Shen, Y Zhou, J Ye, AAA Al-Maskri, Y Kang… - Journal of …, 2020 - Elsevier
The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a
great threat to global public health. Therefore, rapid and accurate identification of …

Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer

H Zhang, C Qin, C An, X Zheng, S Wen, W Chen, X Liu… - Molecular cancer, 2021 - Springer
Abstract The 2020 Nobel Prize in Chemistry was awarded to Emmanuelle Charpentier and
Jennifer Doudna for the development of the Clustered regularly interspaced short …

Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes

C Xu, Y Zhou, Q **ao, B He, G Geng, Z Wang, B Cao… - Nature …, 2021 - nature.com
Competitive coevolution between microbes and viruses has led to the diversification of
CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic …

[HTML][HTML] Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza

TR Abbott, G Dhamdhere, Y Liu, X Lin, L Goudy… - Cell, 2020 - cell.com
Summary The coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-
2 virus, has highlighted the need for antiviral approaches that can target emerging viruses …

Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA

D Wang, S He, X Wang, Y Yan, J Liu, S Wu… - Nature Biomedical …, 2020 - nature.com
Abstract The coronavirus disease 2019 (COVID-19) pandemic has highlighted the need for
rapid and accurate nucleic acid detection at the point of care. Here, we report an …

Electrochemical SARS-CoV-2 sensing at point-of-care and artificial intelligence for intelligent COVID-19 management

AK Kaushik, JS Dhau, H Gohel, YK Mishra… - ACS Applied Bio …, 2020 - ACS Publications
To manage the COVID-19 pandemic, development of rapid, selective, sensitive diagnostic
systems for early stage β-coronavirus severe acute respiratory syndrome (SARS-CoV-2) …

Massively parallel Cas13 screens reveal principles for guide RNA design

HH Wessels, A Méndez-Mancilla, X Guo, M Legut… - Nature …, 2020 - nature.com
Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable
specific and robust target gene knockdown without altering the genome. To define rules for …

Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents

EL Blanchard, D Vanover, SS Bawage, PM Tiwari… - Nature …, 2021 - nature.com
Cas13a has been used to target RNA viruses in cell culture, but efficacy has not been
demonstrated in animal models. In this study, we used messenger RNA (mRNA)-encoded …

Nanotechnology's frontier in combatting infectious and inflammatory diseases: prevention and treatment

Y Huang, X Guo, Y Wu, X Chen, L Feng, N **e… - … and Targeted Therapy, 2024 - nature.com
Inflammation-associated diseases encompass a range of infectious diseases and non-
infectious inflammatory diseases, which continuously pose one of the most serious threats to …

CRISPR technologies for genome, epigenome and transcriptome editing

L Villiger, J Joung, L Koblan, J Weissman… - … Reviews Molecular Cell …, 2024 - nature.com
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …