Cell line development represents a crucial step in the development process of a therapeutic
glycoprotein. Chinese hamster ovary (CHO) cells are the most frequently employed …

Mammalian cells have developed dedicated molecular mechanisms to tightly control
expression levels of their genes where the specific transcriptomic signature across all genes …

Cell line generation of mammalian cells is a time‐consuming and labor‐intensive process,
especially because of challenges in clone selection after transfection. Antibiotics are …

The evolving development landscape of biotherapeutics and their growing complexity from
simple antibodies into bi-and multi-specific molecules necessitates sophisticated discovery …

Oligoclonal antibodies, which are carefully defined mixtures of monoclonal antibodies, are
valuable for the treatment of complex diseases, such as infectionss and cancer. In addition …

The integration of a transgene expression construct into the host genome is the initial step
for the generation of recombinant cell lines used for biopharmaceutical production. The …

Gene therapy is poised to transition from niche to mainstream medicine, with recombinant
adeno-associated virus (rAAV) as the vector of choice. However, robust, scalable …

Chinese hamster ovary (CHO) cells are the most widely used platform for recombinant
therapeutic protein (RTP) production. Traditionally, the development of CHO cell lines has …

In traditional cell line design pipelines, cost-and time-intensive long-term stability studies
must be performed due to random integration of the transgene into the genome. By this …

Introduction The aim of this study was to compare two CRISPR/Cas9-based orthogonal
strategies, paired-Cas9 nickase (paired-Cas9n) and RNA-guided FokI (RFN), in targeting …