Gene therapy has historically been defined as the addition of new genes to human cells.
However, the recent advent of genome-editing technologies has enabled a new paradigm in …

Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of
cell types and organisms. The newly-developed transcription activator-like effector …

Synthetic gene circuits that precisely control human cell function could expand the
capabilities of gene-and cell-based therapies. However, platforms for develo** circuits in …

Genome engineering with programmable nucleases depends on cellular responses to a
targeted double-strand break (DSB). The first truly targetable reagents were the zinc finger …

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for
targeted mutagenesis. Here we report a new class of sequence-specific nucleases created …

DddA-derived cytosine base editors (DdCBEs) use programmable DNA-binding TALE
repeat arrays, rather than CRISPR proteins, a split double-stranded DNA cytidine …

Abstract Cys2-His2 zinc finger (C2H2-ZF) proteins represent the largest class of putative
human transcription factors. However, for most C2H2-ZF proteins it is unknown whether they …

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we
describe context-dependent assembly (CoDA), a platform for engineering ZFNs using only …

Zinc-finger nucleases (ZFNs) are hybrids between a nonspecific DNA-cleavage domain and
a DNA-binding domain composed of Cys2His2 zinc fingers. Because zinc fingers can be …

An account is given of the discovery of the classical Cys2His2 zinc finger, arising from the
interpretation of biochemical studies on the interaction of the Xenopus protein transcription …