RNA sample optimization for cryo-EM analysis

X Chen, L Wang, J **e, JS Nowak, B Luo, C Zhang… - Nature …, 2024 - nature.com
RNAs play critical roles in most biological processes. Although the three-dimensional (3D)
structures of RNAs primarily determine their functions, it remains challenging to …

[HTML][HTML] Challenges, advances, and opportunities in RNA structural biology by Cryo-EM

SL Bonilla, K Jang - Current Opinion in Structural Biology, 2024 - Elsevier
RNAs are remarkably versatile molecules that can fold into intricate three-dimensional (3D)
structures to perform diverse cellular and viral functions. Despite their biological importance …

Conformational penalties: New insights into nucleic acid recognition

A Geng, R Roy, HM Al-Hashimi - Current Opinion in Structural Biology, 2024 - Elsevier
Highlights•Conformational penalties to form alternative conformational states of nucleic
acids greatly contribute to nucleic acid-protein recognition.•Methods to determine nucleic …

[HTML][HTML] Structural and biophysical dissection of RNA conformational ensembles

SL Bonilla, AN Jones, D Incarnato - Current Opinion in Structural Biology, 2024 - Elsevier
RNA's ability to form and interconvert between multiple secondary and tertiary structures is
critical to its functional versatility and the traditional view of RNA structures as static entities …

Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5′-Untranslated Region of SARS-CoV-2

KR Mertinkus, A Oxenfarth, C Richter… - Journal of the …, 2024 - ACS Publications
Throughout the family of coronaviruses, structured RNA elements within the 5′ region of
the genome are highly conserved. The fifth stem-loop element from SARS-CoV-2 (5_SL5) …