Optogenetics combines light and genetics to enable precise control of living cells, tissues,
and organisms with tailored functions. Optogenetics has the advantages of …

Protein knockdown using the auxin-inducible degron (AID) technology is useful to study
protein function in living cells because it induces rapid depletion, which makes it possible to …

In higher eukaryotes, enhancers determine the activation of developmental gene
transcription in specific cell types and stages of embryogenesis. Enhancers transform the …

The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …

Inducible protein knockdowns are excellent tools to test the function of essential proteins in
short time scales and to capture the role of proteins in dynamic events. Current approaches …

Interactions between distal loci, including those involving enhancers and promoters, are a
central mechanism of gene regulation in mammals, yet the protein regulators of these …

Protein kinases regulate fundamental aspects of eukaryotic cell biology, making them
attractive chemotherapeutic targets in parasites like Plasmodium spp. and Toxoplasma …

Conditional degron tags (CDTs) are a powerful tool for target validation that combines the
kinetics and reversible action of pharmacological agents with the generalizability of genetic …

Cardiac contractions and hemodynamic forces are essential for organ development and
homeostasis. Control over cardiac contractions can be achieved pharmacologically or …

Gene expression is orchestrated by transcription factors, which function within the context of
a three-dimensional genome. Zinc-finger protein 143 (ZNF143/ZFP143) is a transcription …