Long-read sequencing (LRS) technologies have provided extremely powerful tools to
explore genomes. While in the early years these methods suffered technical limitations, they …

Chromosomes of eukaryotes adopt highly dynamic and complex hierarchical structures in
the nucleus. The three-dimensional (3D) organization of chromosomes profoundly affects …

Construction of chromosome-level assembly is a vital step in achieving the goal of a
'Platinum'genome, but it remains a major challenge to assemble and anchor sequences to …

Species often include multiple ecotypes that are adapted to different environments.
However, it is unclear how ecotypes arise and how their distinctive combinations of adaptive …

Long-read sequencing and novel long-range assays have revolutionized de novo genome
assembly by automating the reconstruction of reference-quality genomes. In particular, Hi-C …

The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to create high-
quality assemblies of large genomes in a rapid and cost-effective way. Here we combine Hi …

Pangenomes provide access to an accurate representation of the genetic diversity of
species, both in terms of sequence polymorphisms and structural variants (SVs). Here we …

Long-range and highly accurate de novo assembly from short-read data is one of the most
pressing challenges in genomics. Recently, it has been shown that read pairs generated by …

Background Long read technologies have revolutionized de novo genome assembly by
generating contigs orders of magnitude longer than that of short read assemblies. Although …

Here, we report the design, construction, and characterization of a tRNA neochromosome, a
designer chromosome that functions as an additional, de novo counterpart to the native …