A framework for understanding the functions of biomolecular condensates across scales

AS Lyon, WB Peeples, MK Rosen - Nature Reviews Molecular Cell …, 2021 - nature.com
Biomolecular condensates are found throughout eukaryotic cells, including in the nucleus, in
the cytoplasm and on membranes. They are also implicated in a wide range of cellular …

Physical principles underlying the complex biology of intracellular phase transitions

JM Choi, AS Holehouse… - Annual review of …, 2020 - annualreviews.org
Many biomolecular condensates appear to form via spontaneous or driven processes that
have the hallmarks of intracellular phase transitions. This suggests that a common …

Protein cages: from fundamentals to advanced applications

TGW Edwardson, MD Levasseur, S Tetter… - Chemical …, 2022 - ACS Publications
Proteins that self-assemble into polyhedral shell-like structures are useful molecular
containers both in nature and in the laboratory. Here we review efforts to repurpose diverse …

Conformational dynamics of intrinsically disordered proteins regulate biomolecular condensate chemistry

A Abyzov, M Blackledge, M Zweckstetter - Chemical Reviews, 2022 - ACS Publications
Motions in biomolecules are critical for biochemical reactions. In cells, many biochemical
reactions are executed inside of biomolecular condensates formed by ultradynamic …

The emergence of phase separation as an organizing principle in bacteria

CA Azaldegui, AG Vecchiarelli, JS Biteen - Biophysical journal, 2021 - cell.com
Recent investigations in bacteria suggest that membraneless organelles play a crucial role
in the subcellular organization of bacterial cells. However, the biochemical functions and …

Liquid-liquid phase separation of RBGD2/4 is required for heat stress resistance in Arabidopsis

S Zhu, J Gu, J Yao, Y Li, Z Zhang, W **a, Z Wang… - Developmental Cell, 2022 - cell.com
As sessile organisms, plants are highly sensitive to environmental stresses. In response to
stresses, globally repressed translation initiation leads to stress granule (SG) formation …

Formation and function of bacterial organelles

C Greening, T Lithgow - Nature Reviews Microbiology, 2020 - nature.com
Advances in imaging technologies have revealed that many bacteria possess organelles
with a proteomically defined lumen and a macromolecular boundary. Some are bound by a …

Rubisco function, evolution, and engineering

N Prywes, NR Phillips, OT Tuck… - Annual review of …, 2023 - annualreviews.org
Carbon fixation is the process by which CO2 is converted from a gas into biomass. The
Calvin–Benson–Bassham cycle (CBB) is the dominant carbon-consuming pathway on …

Identification of a carbonic anhydrase–Rubisco complex within the alpha-carboxysome

C Blikstad, EJ Dugan, TG Laughlin… - Proceedings of the …, 2023 - National Acad Sciences
Carboxysomes are proteinaceous organelles that encapsulate key enzymes of CO2 fixation—
Rubisco and carbonic anhydrase—and are the centerpiece of the bacterial CO2 …

Structure and assembly of cargo Rubisco in two native α-carboxysomes

T Ni, Y Sun, W Burn, MMJ Al-Hazeem, Y Zhu… - Nature …, 2022 - nature.com
Carboxysomes are a family of bacterial microcompartments in cyanobacteria and
chemoautotrophs. They encapsulate Ribulose 1, 5-bisphosphate carboxylase/oxygenase …