Thiamin is synthesized by most prokaryotes and by eukaryotes such as yeast and plants. In
all cases, the thiazole and pyrimidine moieties are synthesized in separate branches of the …

Protein crystallization remains a key limiting step in the characterization of the atomic
structures of proteins and their complexes by X-ray diffraction methods. Current data indicate …

Growing well‐diffracting crystals constitutes a serious bottleneck in structural biology. A
recently proposed crystallization methodology for “stubborn crystallizers” is to engineer …

Since their discovery in the 1960s as 'osmotic shock-sensitive'transporters, a plethora of so-
called binding protein-dependent (canonical) ATP-binding cassette (ABC) importers has …

The ACT domain is a structural motif in proteins of 70–80 amino acids that is one of a
growing number of different intracellular small molecule binding domains that function in the …

Drug resistance of pathogens has necessitated the identification of novel targets for
antibiotics. Thiamin (vitamin B1) is an essential cofactor for all organisms in its active form …

A strategy of rationally engineering protein surfaces with the aim of obtaining mutants that
are distinctly more susceptible to crystallization than the wild-type protein has previously …

Until recently, protein crystallization has mostly been regarded as a stochastic event over
which the investigator has little or no control. With the dramatic technological advances in …

Highlights•The ACT domain is a 60–70 residue domain with a topology.•The ACT domain is
associated with the allosteric regulation of enzymes of amino-acid metabolism.•ACT …

The crystal structure of the product of the Bacillus subtilis ykuD gene was solved by the
multiwavelength anomalous dispersion (MAD) method and refined using data to 2.0 Å …