The human microbiome substantially affects many aspects of human physiology, including
metabolism, drug interactions and numerous diseases. This realization, coupled with ever …

Next generation sequencing (NGS) technologies have impressively accelerated research in
biological science during the last years by enabling the production of large volumes of …

Objective Stroke is a leading cause of death and disability worldwide. Neuroprotective
approaches have failed in clinical trials, thus warranting therapeutic innovations with …

Amplicon-based marker gene surveys form the basis of most microbiome and other
microbial community studies. Such PCR-based methods have multiple steps, each of which …

Abstract Motivation: The Illumina paired-end sequencing technology can generate reads
from both ends of target DNA fragments, which can subsequently be merged to increase the …

Background Gut microbiota has been suggested to play a role in almost all major diseases
including cardio‐and cerebrovascular diseases. A possible mechanism is the transformation …

The effects and regulatory mechanisms of co-inoculation of plant-growth-promoting
rhizobacteria (PGPRs) and rhizobium in plant-soil systems remain unclear, despite …

DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up
to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for …

Background Illumina paired-end reads are used to analyse microbial communities by
targeting amplicons of the 16S rRNA gene. Publicly available tools are needed to assemble …

Sediment, a special realm in aquatic environments, has high microbial diversity. While there
are numerous reports about the microbial community in marine sediment, freshwater and …