Structures, functions, and mechanisms of filament forming enzymes: a renaissance of enzyme filamentation

CK Park, NC Horton - Biophysical reviews, 2019 - Springer
Filament formation by non-cytoskeletal enzymes has been known for decades, yet only
relatively recently has its wide-spread role in enzyme regulation and biology come to be …

The cytoophidium and its kind: filamentation and compartmentation of metabolic enzymes

JL Liu - Annual review of cell and developmental biology, 2016 - annualreviews.org
Compartmentation is essential for the localization of biological processes within a cell. In
2010, three groups independently reported that cytidine triphosphate synthase (CTPS), a …

Human CTP synthase filament structure reveals the active enzyme conformation

EM Lynch, DR Hicks, M Shepherd, JA Endrizzi… - Nature structural & …, 2017 - nature.com
The universally conserved enzyme CTP synthase (CTPS) forms filaments in bacteria and
eukaryotes. In bacteria, polymerization inhibits CTPS activity and is required for nucleotide …

[HTML][HTML] Filamentation of metabolic enzymes in Saccharomyces cerevisiae

QJ Shen, H Kassim, Y Huang, H Li, J Zhang… - Journal of genetics and …, 2016 - Elsevier
Compartmentation via filamentation has recently emerged as a novel mechanism for
metabolic regulation. In order to identify filament-forming metabolic enzymes systematically …

Coupled structural transitions enable highly cooperative regulation of human CTPS2 filaments

EM Lynch, JM Kollman - Nature structural & molecular biology, 2020 - nature.com
Many enzymes assemble into defined oligomers, providing a mechanism for cooperatively
regulating activity. Recent studies have described a mode of regulation in which enzyme …

Cytoophidium assembly reflects upregulation of IMPDH activity

CC Chang, WC Lin, LM Pai, HS Lee… - Journal of cell …, 2015 - journals.biologists.com
Cytidine triphosphate synthase (CTPS) and inosine monophosphate dehydrogenase
(IMPDH)(both of which have two isoforms) can form fiber-like subcellular structures termed …

Filamentation modulates allosteric regulation of PRPS

HH Hu, GM Lu, CC Chang, Y Li, J Zhong, CJ Guo… - Elife, 2022 - elifesciences.org
Phosphoribosyl pyrophosphate (PRPP) is a key intermediate in the biosynthesis of purine
and pyrimidine nucleotides, histidine, tryptophan, and cofactors NAD and NADP. Abnormal …

Drosophila CTP synthase can form distinct substrate-and product-bound filaments

X Zhou, CJ Guo, HH Hu, J Zhong, Q Sun, D Liu… - Journal of Genetics and …, 2019 - Elsevier
Intracellular compartmentation is a key strategy for the functioning of a cell. In 2010, several
studies revealed that the metabolic enzyme CTP synthase (CTPS) can form filamentous …

Metabolic regulation via enzyme filamentation

GN Aughey, JL Liu - Critical reviews in biochemistry and molecular …, 2016 - Taylor & Francis
Determining the mechanisms of enzymatic regulation is central to the study of cellular
metabolism. Regulation of enzyme activity via polymerization-mediated strategies has been …

CTP synthase forms the cytoophidium in human hepatocellular carcinoma

CC Chang, YM Jeng, M Peng, GD Keppeke… - Experimental Cell …, 2017 - Elsevier
CTP synthase (CTPS) can aggregate into an intracellular macrostructure, the cytoophidium,
in various organisms including human cells. Previous studies have shown that assembly of …