Glycoproteomics

I Bagdonaite, SA Malaker, DA Polasky… - Nature Reviews …, 2022 - nature.com
Protein glycosylation involves the co-translational or post-translational addition of glycans to
proteins and is a crucial protein modification in health and disease. The aim of …

The glycosylation in SARS-CoV-2 and its receptor ACE2

Y Gong, S Qin, L Dai, Z Tian - Signal Transduction and Targeted …, 2021 - nature.com
Abstract Coronavirus disease 2019 (COVID-19), a highly infectious disease caused by
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has infected more than …

Precise, fast and comprehensive analysis of intact glycopeptides and modified glycans with pGlyco3

WF Zeng, WQ Cao, MQ Liu, SM He, PY Yang - Nature Methods, 2021 - nature.com
Great advances have been made in mass spectrometric data interpretation for intact
glycopeptide analysis. However, accurate identification of intact glycopeptides and modified …

A pragmatic guide to enrichment strategies for mass spectrometry–based glycoproteomics

NM Riley, CR Bertozzi, SJ Pitteri - Molecular & Cellular Proteomics, 2021 - ASBMB
Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that …

Detecting diagnostic features in MS/MS spectra of post-translationally modified peptides

DJ Geiszler, DA Polasky, F Yu, AI Nesvizhskii - Nature Communications, 2023 - nature.com
Post-translational modifications are an area of great interest in mass spectrometry-based
proteomics, with a surge in methods to detect them in recent years. However, post …

StrucGP: de novo structural sequencing of site-specific N-glycan on glycoproteins using a modularization strategy

J Shen, L Jia, L Dang, Y Su, J Zhang, Y Xu, B Zhu… - Nature …, 2021 - nature.com
Precision map** of glycans at structural and site-specific level is still one of the most
challenging tasks in the glycobiology field. Here, we describe a modularization strategy for …

Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis

R Kawahara, A Chernykh, K Alagesan, M Bern… - Nature …, 2021 - nature.com
Glycoproteomics is a powerful yet analytically challenging research tool. Software packages
aiding the interpretation of complex glycopeptide tandem mass spectra have appeared, but …

Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation

Z Fang, H Qin, J Mao, Z Wang, N Zhang… - Nature …, 2022 - nature.com
Glycopeptides with unusual glycans or poor peptide backbone fragmentation in tandem
mass spectrometry are unaccounted for in typical site-specific glycoproteomics analysis and …

Glycopeptide database search and de novo sequencing with PEAKS GlycanFinder enable highly sensitive glycoproteomics

W Sun, Q Zhang, X Zhang, NH Tran… - Nature …, 2023 - nature.com
Here we present GlycanFinder, a database search and de novo sequencing tool for the
analysis of intact glycopeptides from mass spectrometry data. GlycanFinder integrates …

Emerging roles of O-glycosylation in regulating protein aggregation, phase separation, and functions

X Li, Y Du, X Chen, C Liu - Current Opinion in Chemical Biology, 2023 - Elsevier
Protein O-glycosylation is widely identified in various proteins involved in diverse biological
processes. Recent studies have demonstrated that O-glycosylation plays crucial and …