Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

A guide to light-sheet fluorescence microscopy for multiscale imaging

RM Power, J Huisken - Nature methods, 2017 - nature.com
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens

AK Glaser, NP Reder, Y Chen, EF McCarty… - Nature biomedical …, 2017 - nature.com
For the 1.7 million patients per year in the US who receive a new cancer diagnosis,
treatment decisions are largely based on histopathological specimen examinations …

Tissue clearing of both hard and soft tissue organs with the PEGASOS method

D **g, S Zhang, W Luo, X Gao, Y Men, C Ma, X Liu… - Cell research, 2018 - nature.com
Tissue clearing technique enables visualization of opaque organs and tissues in 3-
dimensions (3-D) by turning tissue transparent. Current tissue clearing methods are …

Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy

HR Ueda, HU Dodt, P Osten, MN Economo… - Neuron, 2020 - cell.com
Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields
have been combined only recently to facilitate novel experiments and measurements in …

[HTML][HTML] Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity

G Lazzari, V Nicolas, M Matsusaki, M Akashi… - Acta biomaterialia, 2018 - Elsevier
The preclinical drug screening of pancreatic cancer treatments suffers from the absence of
appropriate models capable to reproduce in vitro the heterogeneous tumor …

Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution

T Chakraborty, MK Driscoll, E Jeffery, MM Murphy… - Nature …, 2019 - nature.com
We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables
isotropic, subcellular resolution imaging with high optical sectioning capability and a large …

Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples

Y Chen, S Chauhan, C Gong, H Dayton, C Xu… - Nature Biomedical …, 2024 - nature.com
Light-sheet fluorescence microscopy (LSFM) is a widely used technique for imaging cleared
tissue and living samples. However, high-performance LSFM systems are typically …

Light-sheet microscopy and its potential for understanding developmental processes

Y Wan, K McDole, PJ Keller - Annual review of cell and …, 2019 - annualreviews.org
The ability to visualize and quantitatively measure dynamic biological processes in vivo and
at high spatiotemporal resolution is of fundamental importance to experimental …

Tutorial: practical considerations for tissue clearing and imaging

KR Weiss, FF Voigt, DP Shepherd, J Huisken - Nature protocols, 2021 - nature.com
Tissue clearing has become a powerful technique for studying anatomy and morphology at
scales ranging from entire organisms to subcellular features. With the recent proliferation of …