Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

[HTML][HTML] Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR

ASL Thankamony, JJ Wittmann, M Kaushik… - Progress in nuclear …, 2017 - Elsevier
The field of dynamic nuclear polarization has undergone tremendous developments and
diversification since its inception more than 6 decades ago. In this review we provide an in …

Solid-state NMR: Methods for biological solids

S Ahlawat, KR Mote, NA Lakomek… - Chemical Reviews, 2022 - ACS Publications
In the last two decades, solid-state nuclear magnetic resonance (ssNMR) spectroscopy has
transformed from a spectroscopic technique investigating small molecules and industrial …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

In-cell NMR: Why and how?

FX Theillet, E Luchinat - Progress in Nuclear Magnetic Resonance …, 2022 - Elsevier
NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

DNP‐supported solid‐state NMR spectroscopy of proteins inside mammalian cells

S Narasimhan, S Scherpe… - Angewandte Chemie …, 2019 - Wiley Online Library
Elucidating at atomic level how proteins interact and are chemically modified in cells
represents a leading frontier in structural biology. We have developed a tailored solid‐state …

Highly bioresistant, hydrophilic and rigidly linked trityl-nitroxide biradicals for cellular high-field dynamic nuclear polarization

R Yao, D Beriashvili, W Zhang, S Li, A Safeer… - Chemical …, 2022 - pubs.rsc.org
Cellular dynamic nuclear polarization (DNP) has been an effective means of overcoming the
intrinsic sensitivity limitations of solid-state nuclear magnetic resonance (ssNMR) …

In vivo assembly of artificial metalloenzymes and application in whole‐cell biocatalysis

S Chordia, S Narasimhan… - Angewandte Chemie …, 2021 - Wiley Online Library
We report the supramolecular assembly of artificial metalloenzymes (ArMs), based on the
Lactococcal multidrug resistance regulator (LmrR) and an exogeneous copper (II) …

High-field dynamic nuclear polarization

B Corzilius - Annual review of physical chemistry, 2020 - annualreviews.org
Dynamic nuclear polarization (DNP) is one of the most prominent methods of sensitivity
enhancement in nuclear magnetic resonance (NMR). Even though solid-state DNP under …

In-cell NMR: a topical review

E Luchinat, L Banci - IUCrJ, 2017 - journals.iucr.org
Classical structural biology approaches allow structural characterization of biological
macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth …