The observation of molecular diffusion at different spatial scales, and in particular below the
optical diffraction limit (< 200 nm), can reveal details of the subcellular topology and its …

We have implemented scanning fluorescence correlation spectroscopy (sFCS) for precise
determination of diffusion coefficients of fluorescent molecules in solution. The measurement …

This chapter presents a review of fluorescence correlation spectroscopy (FCS), an
experimental technique with single-molecule sensitivity, which is based on the analysis of …

We demonstrate that channelrhodopsin-2 (CR), a light-gated ion channel that is
conventionally activated by using visible-light excitation, can also be activated by using IR …

Two-photon probe excitation data are commonly presented as absorption cross section or
molecular brightness (the detected fluorescence rate per molecule). We report two-photon …

Complex diffusive dynamics are often observed when one is investigating the mobility of
macromolecules in living cells and other complex environments, yet the underlying physical …

Measuring protein interactions is key to understanding cell signaling mechanisms, but
quantitative analysis of these interactions in situ has remained a major challenge. Here, we …

The quaternary organization of rhodopsin-like G protein-coupled receptors in native tissues
is unknown. To address this we generated mice in which the M1 muscarinic acetylcholine …

Abstract Development of biologically relevant crowding solutions necessitates improved
understanding of how the relative size and density of mobile obstacles affect probe diffusion …

Fast synaptic communication uses diffusible transmitters whose spread is limited by uptake
mechanisms. However, on the submicron-scale, the distance between two synapses, the …