Biotechnology applications of cell-free expression systems
Cell-free systems are a rapidly expanding platform technology with an important role in the
engineering of biological systems. The key advantages that drive their broad adoption are …
engineering of biological systems. The key advantages that drive their broad adoption are …
Cell-free gene expression: methods and applications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …
molecules and processes outside of living intact cells. The foundational principle is that …
Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples
M Karlikow, SJR da Silva, Y Guo, S Cicek… - Nature Biomedical …, 2022 - nature.com
In low-resource settings, resilience to infectious disease outbreaks can be hindered by
limited access to diagnostic tests. Here we report the results of double-blinded studies of the …
limited access to diagnostic tests. Here we report the results of double-blinded studies of the …
A low-cost, thermostable, cell-free protein synthesis platform for on-demand production of conjugate vaccines
Cell-free protein synthesis systems that can be lyophilized for long-term, non-refrigerated
storage and transportation have the potential to enable decentralized biomanufacturing …
storage and transportation have the potential to enable decentralized biomanufacturing …
Constructing cell-free expression systems for low-cost access
Cell-free systems for gene expression have gained attention as platforms for the facile study
of genetic circuits and as highly effective tools for teaching. Despite recent progress, the …
of genetic circuits and as highly effective tools for teaching. Despite recent progress, the …
Bacterial Metallostasis: Metal Sensing, Metalloproteome Remodeling, and Metal Trafficking
Transition metals function as structural and catalytic cofactors for a large diversity of proteins
and enzymes that collectively comprise the metalloproteome. Metallostasis considers all …
and enzymes that collectively comprise the metalloproteome. Metallostasis considers all …
Advancing biomimetic functions of synthetic cells through compartmentalized cell-free protein synthesis
Synthetic cells are artificial constructs that mimic the structures and functions of living cells.
They are attractive for studying diverse biochemical processes and elucidating the origins of …
They are attractive for studying diverse biochemical processes and elucidating the origins of …
Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application
Continued waves, new variants, and limited vaccine deployment mean that SARS-CoV-2
tests remain vital to constrain the coronavirus disease 2019 (COVID-19) pandemic …
tests remain vital to constrain the coronavirus disease 2019 (COVID-19) pandemic …
Expanding luciferase reporter systems for cell-free protein expression
Luciferases are often used as a sensitive, versatile reporter in cell-free transcription-
translation (TXTL) systems, for research and practical applications such as engineering …
translation (TXTL) systems, for research and practical applications such as engineering …
Differentially optimized cell-free buffer enables robust expression from unprotected linear DNA in exonuclease-deficient extracts
The use of linear DNA templates in cell-free systems promises to accelerate the prototy**
and engineering of synthetic gene circuits. A key challenge is that linear templates are …
and engineering of synthetic gene circuits. A key challenge is that linear templates are …