Molecular probes, chemosensors, and nanosensors for optical detection of biorelevant molecules and ions in aqueous media and biofluids
Synthetic molecular probes, chemosensors, and nanosensors used in combination with
innovative assay protocols hold great potential for the development of robust, low-cost, and …
innovative assay protocols hold great potential for the development of robust, low-cost, and …
Light sheet fluorescence microscopy
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …
Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …
development of new microscopes, fluorescent labels and analysis techniques has pushed …
Fluorescent proteins and genetically encoded biosensors
M Wang, Y Da, Y Tian - Chemical Society Reviews, 2023 - pubs.rsc.org
The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …
light. They are powerful tools for the visualisation of physiological processes in living cells …
FRET as a biomolecular research tool—understanding its potential while avoiding pitfalls
The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …
However, different FRET techniques are not applied consistently, nor are results uniformly …
De novo design of modular and tunable protein biosensors
Naturally occurring protein switches have been repurposed for the development of
biosensors and reporters for cellular and clinical applications. However, the number of such …
biosensors and reporters for cellular and clinical applications. However, the number of such …
Structure-and mechanism-guided design of single fluorescent protein-based biosensors
Intensiometric genetically encoded biosensors, based on allosteric modulation of the
fluorescence of a single fluorescent protein, are powerful tools for enabling imaging of …
fluorescence of a single fluorescent protein, are powerful tools for enabling imaging of …
Optogenetics at the presynapse
Optogenetic actuators enable highly precise spatiotemporal interrogation of biological
processes at levels ranging from the subcellular to cells, circuits and behaving organisms …
processes at levels ranging from the subcellular to cells, circuits and behaving organisms …
[HTML][HTML] Phase separation of a PKA regulatory subunit controls cAMP compartmentation and oncogenic signaling
The fidelity of intracellular signaling hinges on the organization of dynamic activity
architectures. Spatial compartmentation was first proposed over 30 years ago to explain how …
architectures. Spatial compartmentation was first proposed over 30 years ago to explain how …
A high-throughput multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors
Genetically encoded fluorescent biosensors are powerful tools used to track chemical
processes in intact biological systems. However, the development and optimization of …
processes in intact biological systems. However, the development and optimization of …