Porcine model for the study of sperm capacitation, fertilization and male fertility

M Zigo, P Maňásková-Postlerová, D Zuidema… - Cell and tissue …, 2020 - Springer
Mammalian fertilization remains a poorly understood event with the vast majority of studies
done in the mouse model. The purpose of this review is to revise the current knowledge …

Ligands and receptors involved in the sperm-zona pellucida interactions in mammals

L Tumova, M Zigo, P Sutovsky, M Sedmikova… - Cells, 2021 - mdpi.com
Sperm-zona pellucida (ZP) interaction, involving the binding of sperm surface ligands to
complementary carbohydrates of ZP, is the first direct gamete contact event crucial for …

Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability

FP Gomes, R Park, AG Viana, C Fernandez-Costa… - Scientific Reports, 2020 - nature.com
The present study investigated the seminal plasma proteome of Holstein bulls with low (LF;
n= 6) and high (HF; n= 8) sperm freezability. The percentage of viable frozen-thawed sperm …

Zinc is a master-regulator of sperm function associated with binding, motility, and metabolic modulation during porcine sperm capacitation

M Zigo, K Kerns, S Sen, C Essien, R Oko, D Xu… - Communications …, 2022 - nature.com
Sperm capacitation is a post-testicular maturation step endowing spermatozoa with
fertilizing capacity within the female reproductive tract, significant for fertility, reproductive …

Sperm cohort-specific zinc signature acquisition and capacitation-induced zinc flux regulate sperm-oviduct and sperm-zona pellucida interactions

K Kerns, M Sharif, M Zigo, W Xu, LE Hamilton… - International journal of …, 2020 - mdpi.com
Building on our recent discovery of the zinc signature phenomenon present in boar, bull,
and human spermatozoa, we have further characterized the role of zinc ions in the …

Compartmentalization of the proteasome-interacting proteins during sperm capacitation

M Zigo, P Manaskova-Postlerova, V Jonakova… - Scientific Reports, 2019 - nature.com
Ubiquitination is a stable, reversible posttranslational modification of target proteins by
covalent ligation of the small chaperone protein ubiquitin. Most commonly ubiquitination …

Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans

M Sharif, V Hickl, G Juarez, X Di, K Kerns, P Sutovsky… - Scientific reports, 2022 - nature.com
Fertilizing sperm are retained by adhesion to specific glycans on the epithelium of the
oviduct forming a reservoir before sperm are released from the reservoir so fertilization can …

Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig

SM Toledo-Guardiola, C Luongo… - Frontiers in Cell and …, 2023 - frontiersin.org
The seminal plasma (SP) is the liquid component of semen that facilitates sperm transport
through the female genital tract. SP modulates the activity of the ovary, oviductal …

The ubiquitin-proteasome system participates in sperm surface subproteome remodeling during boar sperm capacitation

M Zigo, K Kerns, P Sutovsky - Biomolecules, 2023 - mdpi.com
Sperm capacitation is a complex process endowing biological and biochemical changes to
a spermatozoon for a successful encounter with an oocyte. The present study focused on the …

Assessments of sperm quality integrating morphology, swimming patterns, bioenergetics and cell signalling

ERS Roldan - Theriogenology, 2020 - Elsevier
Spermatozoa are diverse in form and function and these differences impact on their
fertilizing capacity. Because of considerable inter-male and inter-species differences in …