Soybean is one of the most important sources of plant protein and is known for its wide
range of agricultural, food, and industrial applications as well as health benefits. Interest in …

Clathrin-coated vesicles (CCVs) are formed at the plasma membrane and act as vectors for
endocytosis. They also assemble at the trans-Golgi network (TGN), but their exact function at …

The pH of intracellular compartments is essential for the viability of cells. Despite its
relevance, little is known about the pH of these compartments. To measure pH in vivo, we …

The plant trans-Golgi network/early endosome (TGN/EE) is a major hub for secretory and
endocytic trafficking with complex molecular mechanisms controlling sorting and transport of …

The endomembrane system is a complex and dynamic intracellular trafficking network. It is
very challenging to track individual vesicles and their cargos in real time; however, affinity …

The pH homeostasis of endomembranes is essential for cellular functions. In order to
provide direct pH measurements in the endomembrane system lumen, we targeted …

Protein trafficking requires proper ion and pH homeostasis of the endomembrane system.
The NHX-type Na+/H+ antiporters NHX5 and NHX6 localize to the Golgi, trans-Golgi …

Plant pathogens deliver effectors into plant cells to suppress immunity. Whereas many
effectors inactivate positive immune regulators, other effectors associate with negative …

Vacuoles perform a multitude of functions in plant cells, including the storage of amino acids
and sugars. Tonoplast-localized transporters catalyze the import and release of these …

The sorting of soluble proteins for degradation in the vacuole is of vital importance in plant
cells, and relies on the activity of vacuolar sorting receptors (VSRs). In the plant …