Illuminating entomological dark matter with DNA barcodes in an era of insect decline, deep learning, and genomics

R Meier, MKN Lawniczak… - Annual Review of …, 2025 - annualreviews.org
Most insects encountered in the field are initially entomological dark matter in that they
cannot be identified to species while alive. This explains the enduring quest for efficient …

Harnessing the omics revolution to address the global biodiversity crisis

LF De León, B Silva, KJ Avilés-Rodríguez… - Current Opinion in …, 2023 - Elsevier
Highlights•Human disturbances are driving the current biodiversity crisis.•Fundamental
biodiversity challenges include biodiversity discovery, loss, and preservation.•The omics …

Barcode 100k specimens: in a single nanopore run

PDN Hebert, R Floyd, S Jafarpour… - Molecular Ecology …, 2025 - Wiley Online Library
It is a global priority to better manage the biosphere, but action must be informed by
comprehensive data on the abundance and distribution of species. The acquisition of such …

DNA metabarcoding and morphological identification reveal similar richness, taxonomic composition and body size patterns among flying insect communities

N Remmel, D Buchner, J Enss… - Insect Conservation …, 2024 - Wiley Online Library
Tracking insect biodiversity trends and predicting future trajectories is limited by a lack of
monitoring data across large spatiotemporal scales. DNA metabarcoding is a time‐and cost …

Degree of urbanization and vegetation type shape soil biodiversity in city parks

H Yao, Z Li, S Geisen, Z Qiao, MF Breed… - Science of the Total …, 2023 - Elsevier
Urbanization negatively impacts aboveground biodiversity, such as bird and insect
communities. City parks can reduce these negative impacts by providing important habitat …

Metabarcoding advances agricultural invertebrate biomonitoring by enhancing resolution, increasing throughput and facilitating network inference

BSJ Hawthorne, JP Cuff, LE Collins… - Agricultural and Forest …, 2025 - Wiley Online Library
Biomonitoring of agriculturally important insects is increasingly vital given our need to
understand:(a) the severity of impacts by pests and pathogens on crop yield and health and …

Illuminating arthropod diversity in a tropical forest: Assessing biodiversity by automatic light trap** and DNA metabarcoding

D Souto‐Vilarós, Y Basset, P Blažek… - Environmental …, 2024 - Wiley Online Library
Although studies of insect decline have recently dominated headlines worldwide, their
interpretation requires caution since for most species, we lack long‐term population …

Metabarcoding: opportunities for accelerating monitoring and understanding insect tropical biodiversity

EJ Alvarado-Robledo… - Journal of Insect …, 2024 - Springer
Despite occupying only a small portion of the Earth's surface, tropical rainforests are some of
the most diverse ecosystems on the planet. Given the hyperdiversity of tropical rainforests …

[HTML][HTML] Ecological monitoring using Collembola metabarcoding with extremely low bycatch amplification

PM Pedro, L Cullen Jr, F Prado, A Uezu, R Piper… - Ecological …, 2023 - Elsevier
Collembola are used widely to monitor soil health and functional parameters. Recent
developments in high throughput sequencing (especially metabarcoding) have substantially …

Metabarcoding of a mock community of soil invertebrates: DNA extraction, false positives, and data filtration

IV Sotnikov, MV Vecherskii, VS Cheptsov, AV Tiunov - Pedobiologia, 2025 - Elsevier
Metabarcoding is a powerful tool widely used to analyze soil communities but
methodological difficulties can introduce biases, resulting in inaccurate estimation of species …