Recombinant protein expression in Escherichia coli: advances and challenges

GL Rosano, EA Ceccarelli - Frontiers in microbiology, 2014 - frontiersin.org
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems

K Terpe - Applied microbiology and biotechnology, 2006 - Springer
During the proteomics period, the growth in the use of recombinant proteins has increased
greatly in the recent years. Bacterial systems remain most attractive due to low cost, high …

Gut inflammation provides a respiratory electron acceptor for Salmonella

SE Winter, P Thiennimitr, MG Winter, BP Butler… - Nature, 2010 - nature.com
Salmonella enterica serotype Typhimurium (S. Typhimurium) causes acute gut inflammation
by using its virulence factors to invade the intestinal epithelium and survive in mucosal …

Precision editing of the gut microbiota ameliorates colitis

W Zhu, MG Winter, MX Byndloss, L Spiga, BA Duerkop… - Nature, 2018 - nature.com
Inflammatory diseases of the gastrointestinal tract are frequently associated with
dysbiosis,,,,,,,, characterized by changes in gut microbial communities that include an …

Host-Derived Nitrate Boosts Growth of E. coli in the Inflamed Gut

SE Winter, MG Winter, MN Xavier, P Thiennimitr… - science, 2013 - science.org
Changes in the microbial community structure are observed in individuals with intestinal
inflammatory disorders. These changes are often characterized by a depletion of obligate …

Mucispirillum schaedleri antagonizes Salmonella virulence to protect mice against colitis

S Herp, S Brugiroux, D Garzetti, D Ring, LM Jochum… - Cell host & …, 2019 - cell.com
The microbiota and the gastrointestinal mucus layer play a pivotal role in protection against
non-typhoidal Salmonella enterica serovar Typhimurium (S. Tm) colitis. Here, we analyzed …

Release of outer membrane vesicles by Gram‐negative bacteria is a novel envelope stress response

AJ McBroom, MJ Kuehn - Molecular microbiology, 2007 - Wiley Online Library
Conditions that impair protein folding in the Gram‐negative bacterial envelope cause stress.
The destabilizing effects of stress in this compartment are recognized and countered by a …

Caspase-11 protects against bacteria that escape the vacuole

Y Aachoui, IA Leaf, JA Hagar, MF Fontana… - Science, 2013 - science.org
Caspases are either apoptotic or inflammatory. Among inflammatory caspases, caspase-1
and-11 trigger pyroptosis, a form of programmed cell death. Whereas both can be …

Amplifying genetic logic gates

J Bonnet, P Yin, ME Ortiz, P Subsoontorn, D Endy - Science, 2013 - science.org
Organisms must process information encoded via developmental and environmental signals
to survive and reproduce. Researchers have also engineered synthetic genetic logic to …

PmrA–PmrB‐regulated genes necessary for 4‐aminoarabinose lipid A modification and polymyxin resistance

JS Gunn, KB Lim, J Krueger, K Kim, L Guo… - Molecular …, 1998 - Wiley Online Library
Antimicrobial peptides are distributed throughout the animal kingdom and are a key
component of innate immunity. Salmonella typhimurium regulates mechanisms of resistance …