[HTML][HTML] Protein oxidation-Formation mechanisms, detection and relevance as biomarkers in human diseases

R Kehm, T Baldensperger, J Raupbach, A Höhn - Redox Biology, 2021 - Elsevier
Generation of reactive oxygen species and related oxidants is an inevitable consequence of
life. Proteins are major targets for oxidation reactions, because of their rapid reaction rates …

Mass spectrometry methods for measuring protein stability

DD Vallejo, C Rojas Ramírez, KF Parson, Y Han… - Chemical …, 2022 - ACS Publications
Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

Mass spectrometry techniques in emerging pathogens studies: COVID-19 perspectives

I Mahmud, TJ Garrett - Journal of the American Society for Mass …, 2020 - ACS Publications
As corona virus disease 2019 (COVID-19) is a rapidly growing public health crisis across the
world, our knowledge of meaningful diagnostic tests and treatment for severe acute …

Integration of mass spectrometry data for structural biology

HM Britt, T Cragnolini, K Thalassinos - Chemical Reviews, 2021 - ACS Publications
Mass spectrometry (MS) is increasingly being used to probe the structure and dynamics of
proteins and the complexes they form with other macromolecules. There are now several …

Added value of internal fragments for top-down mass spectrometry of intact monoclonal antibodies and antibody–drug conjugates

B Wei, C Lantz, W Liu, R Viner… - Analytical …, 2023 - ACS Publications
Monoclonal antibodies (mAbs) and antibody–drug conjugates (ADCs) are two of the most
important therapeutic drug classes that require extensive characterization, whereas their …

Seeing the complete picture: proteins in top-down mass spectrometry

T Habeck, F Lermyte - Essays in Biochemistry, 2023 - portlandpress.com
Top-down protein mass spectrometry can provide unique insights into protein sequence and
structure, including precise proteoform identification and study of protein–ligand and protein …

Structural and functional characterization of SARS-CoV-2 RBD domains produced in mammalian cells

C Gstottner, T Zhang, A Resemann, S Ruben… - Analytical …, 2021 - ACS Publications
As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still
ongoing and dramatically influences our life, the need for recombinant viral proteins for …

Progress and challenges in mass spectrometry-based analysis of antibody repertoires

I Snapkov, M Chernigovskaya, P Sinitcyn… - Trends in …, 2022 - cell.com
Humoral immunity is divided into the cellular B cell and protein-level antibody responses.
High-throughput sequencing has advanced our understanding of both these fundamental …

A perspective toward mass spectrometry-based de novo sequencing of endogenous antibodies

SC de Graaf, M Hoek, S Tamara, AJR Heck - MAbs, 2022 - Taylor & Francis
ABSTRACT A key step in therapeutic and endogenous humoral antibody characterization is
identifying the amino acid sequence. So far, this task has been mainly tackled through …

ClipsMS: an algorithm for analyzing internal fragments resulting from top-down mass spectrometry

C Lantz, MA Zenaidee, B Wei… - Journal of proteome …, 2021 - ACS Publications
Top-down mass spectrometry (TD-MS) of peptides and proteins results in product ions that
can be correlated to polypeptide sequence. Fragments can either be terminal fragments …