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X-ray computed tomography in life sciences
Recent developments within micro-computed tomography (μCT) imaging have combined to
extend our capacity to image tissue in three (3D) and four (4D) dimensions at micron and …
extend our capacity to image tissue in three (3D) and four (4D) dimensions at micron and …
Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells
Cryo-soft X-ray tomography is an imaging technique that addresses the need for mesoscale
imaging of cellular ultrastructure of relatively thick samples without the need for staining or …
imaging of cellular ultrastructure of relatively thick samples without the need for staining or …
3D correlative cryo-structured illumination fluorescence and soft X-ray microscopy elucidates reovirus intracellular release pathway
Imaging of biological matter across resolution scales entails the challenge of preserving the
direct and unambiguous correlation of subject features from the macroscopic to the …
direct and unambiguous correlation of subject features from the macroscopic to the …
Structural basis of vesicle formation at the inner nuclear membrane
Vesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular
mechanism for translocation of large cargoes across the nuclear envelope. Cargo is …
mechanism for translocation of large cargoes across the nuclear envelope. Cargo is …
Material and elastic properties of Al‐tobermorite in ancient Roman seawater concrete
The material characteristics and elastic properties of aluminum‐substituted 11 Å tobermorite
in the relict lime clasts of 2000‐year‐old Roman seawater harbor concrete are described …
in the relict lime clasts of 2000‐year‐old Roman seawater harbor concrete are described …
Towards correlative super‐resolution fluorescence and electron cryo‐microscopy
G Wolff, C Hagen, K Grünewald… - Biology of the Cell, 2016 - Wiley Online Library
Correlative light and electron microscopy (CLEM) has become a powerful tool in life
sciences. Particularly cryo‐CLEM, the combination of fluorescence cryo‐microscopy (cryo …
sciences. Particularly cryo‐CLEM, the combination of fluorescence cryo‐microscopy (cryo …
Three-dimensional super-resolution protein localization correlated with vitrified cellular context
We demonstrate the use of cryogenic super-resolution correlative light and electron
microscopy (csCLEM) to precisely determine the spatial relationship between proteins and …
microscopy (csCLEM) to precisely determine the spatial relationship between proteins and …
Super-resolution microscopy using standard fluorescent proteins in intact cells under cryo-conditions
R Kaufmann, P Schellenberger, E Seiradake… - Nano …, 2014 - ACS Publications
We introduce a super-resolution technique for fluorescence cryo-microscopy based on
photoswitching of standard genetically encoded fluorescent marker proteins in intact …
photoswitching of standard genetically encoded fluorescent marker proteins in intact …
Compact x-ray microscope for the water window based on a high brightness laser plasma source
H Legall, G Blobel, H Stiel, W Sandner, C Seim… - Optics express, 2012 - opg.optica.org
We present a laser plasma based x-ray microscope for the water window employing a high-
average power laser system for plasma generation. At 90 W laser power a brightness of 7.4 …
average power laser system for plasma generation. At 90 W laser power a brightness of 7.4 …
[HTML][HTML] Imaging endosomes and autophagosomes in whole mammalian cells using correlative cryo-fluorescence and cryo-soft X-ray microscopy (cryo-CLXM)
EMH Duke, M Razi, A Weston, P Guttmann, S Werner… - Ultramicroscopy, 2014 - Elsevier
Cryo-soft X-ray tomography (cryo-SXT) is a powerful imaging technique that can extract
ultrastructural information from whole, unstained mammalian cells as close to the living state …
ultrastructural information from whole, unstained mammalian cells as close to the living state …