X-ray computed tomography in life sciences

SD Rawson, J Maksimcuka, PJ Withers, SH Cartmell - BMC biology, 2020 - Springer
Recent developments within micro-computed tomography (μCT) imaging have combined to
extend our capacity to image tissue in three (3D) and four (4D) dimensions at micron and …

Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells

M Harkiolaki, MC Darrow, MC Spink… - Emerging topics in …, 2018 - portlandpress.com
Cryo-soft X-ray tomography is an imaging technique that addresses the need for mesoscale
imaging of cellular ultrastructure of relatively thick samples without the need for staining or …

3D correlative cryo-structured illumination fluorescence and soft X-ray microscopy elucidates reovirus intracellular release pathway

I Kounatidis, ML Stanifer, MA Phillips, P Paul-Gilloteaux… - Cell, 2020 - cell.com
Imaging of biological matter across resolution scales entails the challenge of preserving the
direct and unambiguous correlation of subject features from the macroscopic to the …

Structural basis of vesicle formation at the inner nuclear membrane

C Hagen, KC Dent, T Zeev-Ben-Mordehai, M Grange… - Cell, 2015 - cell.com
Vesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular
mechanism for translocation of large cargoes across the nuclear envelope. Cargo is …

Material and elastic properties of Al‐tobermorite in ancient Roman seawater concrete

MD Jackson, J Moon, E Gotti, R Taylor… - Journal of the …, 2013 - Wiley Online Library
The material characteristics and elastic properties of aluminum‐substituted 11 Å tobermorite
in the relict lime clasts of 2000‐year‐old Roman seawater harbor concrete are described …

Towards correlative super‐resolution fluorescence and electron cryo‐microscopy

G Wolff, C Hagen, K Grünewald… - Biology of the Cell, 2016 - Wiley Online Library
Correlative light and electron microscopy (CLEM) has become a powerful tool in life
sciences. Particularly cryo‐CLEM, the combination of fluorescence cryo‐microscopy (cryo …

Three-dimensional super-resolution protein localization correlated with vitrified cellular context

B Liu, Y Xue, W Zhao, Y Chen, C Fan, L Gu, Y Zhang… - Scientific reports, 2015 - nature.com
We demonstrate the use of cryogenic super-resolution correlative light and electron
microscopy (csCLEM) to precisely determine the spatial relationship between proteins and …

Super-resolution microscopy using standard fluorescent proteins in intact cells under cryo-conditions

R Kaufmann, P Schellenberger, E Seiradake… - Nano …, 2014 - ACS Publications
We introduce a super-resolution technique for fluorescence cryo-microscopy based on
photoswitching of standard genetically encoded fluorescent marker proteins in intact …

Compact x-ray microscope for the water window based on a high brightness laser plasma source

H Legall, G Blobel, H Stiel, W Sandner, C Seim… - Optics express, 2012 - opg.optica.org
We present a laser plasma based x-ray microscope for the water window employing a high-
average power laser system for plasma generation. At 90 W laser power a brightness of 7.4 …

[HTML][HTML] Imaging endosomes and autophagosomes in whole mammalian cells using correlative cryo-fluorescence and cryo-soft X-ray microscopy (cryo-CLXM)

EMH Duke, M Razi, A Weston, P Guttmann, S Werner… - Ultramicroscopy, 2014 - Elsevier
Cryo-soft X-ray tomography (cryo-SXT) is a powerful imaging technique that can extract
ultrastructural information from whole, unstained mammalian cells as close to the living state …