Tuning Promoter Strength through RNA Polymerase Binding Site Design in Escherichia coli
One of the paramount goals of synthetic biology is to have the ability to tune transcriptional
networks to targeted levels of expression at will. As a step in that direction, we have …
networks to targeted levels of expression at will. As a step in that direction, we have …
Comprehensive profiling of diverse genetic reporters with application to whole-cell and cell-free biosensors
Whole-cell and cell-free transcription-translation biosensors have recently become favorable
alternatives to conventional detection methods, as they are cost-effective, environmental …
alternatives to conventional detection methods, as they are cost-effective, environmental …
Distinct mechanisms coordinate transcription and translation under carbon and nitrogen starvation in Escherichia coli
Bacteria adapt to environmental stress by producing proteins that provide stress protection.
However, stress can severely perturb the kinetics of gene expression, disrupting protein …
However, stress can severely perturb the kinetics of gene expression, disrupting protein …
Mechanisms of drug interactions between translation-inhibiting antibiotics
B Kavčič, G Tkačik, T Bollenbach - Nature communications, 2020 - nature.com
Antibiotics that interfere with translation, when combined, interact in diverse and difficult-to-
predict ways. Here, we explain these interactions by “translation bottlenecks”: points in the …
predict ways. Here, we explain these interactions by “translation bottlenecks”: points in the …
The highly rugged yet navigable regulatory landscape of the bacterial transcription factor TetR
Transcription factor binding sites (TFBSs) are important sources of evolutionary innovations.
Understanding how evolution navigates the sequence space of such sites can be achieved …
Understanding how evolution navigates the sequence space of such sites can be achieved …
[HTML][HTML] Tuning transcriptional regulation through signaling: a predictive theory of allosteric induction
Allosteric regulation is found across all domains of life, yet we still lack simple, predictive
theories that directly link the experimentally tunable parameters of a system to its input …
theories that directly link the experimentally tunable parameters of a system to its input …
Tunable transcription factor library for robust quantification of regulatory properties in Escherichia coli
Predicting the quantitative regulatory function of transcription factors (TFs) based on factors
such as binding sequence, binding location, and promoter type is not possible. The …
such as binding sequence, binding location, and promoter type is not possible. The …
Operator sequence alters gene expression independently of transcription factor occupancy in bacteria
A canonical quantitative view of transcriptional regulation holds that the only role of operator
sequence is to set the probability of transcription factor binding, with operator occupancy …
sequence is to set the probability of transcription factor binding, with operator occupancy …
Local genetic context shapes the function of a gene regulatory network
Gene expression levels are influenced by multiple coexisting molecular mechanisms. Some
of these interactions such as those of transcription factors and promoters have been studied …
of these interactions such as those of transcription factors and promoters have been studied …
Elimination of carbon catabolite repression in Clostridium acetobutylicum—a journey toward simultaneous use of xylose and glucose
M Bruder, M Moo-Young, DA Chung… - Applied microbiology and …, 2015 - Springer
The industrial Gram-positive anaerobe Clostridium acetobutylicum is a valued acetone,
butanol, and ethanol (ABE) solvent producer that is able to utilize a vast array of carbon …
butanol, and ethanol (ABE) solvent producer that is able to utilize a vast array of carbon …