Fluorescent proteins and their applications in imaging living cells and tissues
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
Green fluorescent protein (GFP): applications, structure, and related photophysical behavior
M Zimmer - Chemical reviews, 2002 - ACS Publications
In the last 10 years green fluorescent protein (GFP) has changed from a nearly unknown
protein to a commonly used tool in molecular biology, medicine, and cell biology. GFP is …
protein to a commonly used tool in molecular biology, medicine, and cell biology. GFP is …
Detection technologies in proteome analysis
WF Patton - Journal of Chromatography B, 2002 - Elsevier
Common strategies employed for general protein detection include organic dye, silver stain,
radiolabeling, reverse stain, fluorescent stain, chemiluminescent stain and mass …
radiolabeling, reverse stain, fluorescent stain, chemiluminescent stain and mass …
Fluorescence in nanobiotechnology: sophisticated fluorophores for novel applications
B Hötzer, IL Medintz, N Hildebrandt - Small, 2012 - Wiley Online Library
Nanobiotechnology is one of the fastest growing and broadest‐ranged interdisciplinary
subfields of the nanosciences. Countless hybrid bio‐inorganic composites are currently …
subfields of the nanosciences. Countless hybrid bio‐inorganic composites are currently …
Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes
Green fluorescent protein (GFP) from jellyfish Aequorea victoria is the most extensively
studied and widely used in cell biology protein. GFP-like proteins constitute a fast growing …
studied and widely used in cell biology protein. GFP-like proteins constitute a fast growing …
Rapid purification of EGFP, EYFP, and ECFP with high yield and purity
SR McRae, CL Brown, GR Bushell - Protein expression and purification, 2005 - Elsevier
Most current high throughput purification procedures for the green fluorescent protein (GFP)
suffer from poor yields and low purity. An improved purification procedure that delivers …
suffer from poor yields and low purity. An improved purification procedure that delivers …
Aqueous biphasic systems composed of cholinium chloride and polymers as effective platforms for the purification of recombinant green fluorescent protein
Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker;
however, its widespread use is limited by its purification costs. Alternative low-cost …
however, its widespread use is limited by its purification costs. Alternative low-cost …
Generation of T-DNA tagging lines with a bidirectional gene trap vector and the establishment of an insertion-site database
We have developed a binary T-DNA vector, pGA2717, that contains the promoter-less β-
glucuronidase (gus) gene adjacent to the right border and the promoter-less green …
glucuronidase (gus) gene adjacent to the right border and the promoter-less green …
An environmentally friendly deproteinization and decolorization method for polysaccharides of Typha angustifolia based on a metal ion-chelating resin adsorption
Y Shi, Z Yuan, T Xu, R Qu, J Yuan, F Cai… - Industrial Crops and …, 2019 - Elsevier
This study developed an environmentally friendly method for the deproteinization and
decolorization of polysaccharide (PTA) from Typha angustifolia using a metal ion-chelating …
decolorization of polysaccharide (PTA) from Typha angustifolia using a metal ion-chelating …
Purification of recombinant green fluorescent protein by three-phase partitioning
The technique of three-phase partitioning (TPP) was used to purify the green fluorescent
protein (GFP) in a single step. TPP uses a combination of ammonium sulphate and tert …
protein (GFP) in a single step. TPP uses a combination of ammonium sulphate and tert …