The expanding role of DNA barcodes: Indispensable tools for ecology, evolution, and conservation

MR Gostel, WJ Kress - Diversity, 2022 - mdpi.com
DNA barcoding has transformed the fields of ecology, evolution, and conservation by
providing a rapid and effective tool for species identification. The growth of DNA barcodes as …

A pile of pipelines: An overview of the bioinformatics software for metabarcoding data analyses

A Hakimzadeh, A Abdala Asbun… - Molecular Ecology …, 2024 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding has gained growing attention as a strategy for
monitoring biodiversity in ecology. However, taxa identifications produced through …

MIDORI2: A collection of quality controlled, preformatted, and regularly updated reference databases for taxonomic assignment of eukaryotic mitochondrial sequences

M Leray, N Knowlton, RJ Machida - Environmental DNA, 2022 - Wiley Online Library
Abstract Analysis of environmental DNA is increasingly used to characterize ecological
communities, but the effectiveness of this approach depends on the accuracy of taxonomic …

Upscaling biodiversity monitoring: Metabarcoding estimates 31,846 insect species from Malaise traps across Germany

D Buchner, JS Sinclair, M Ayasse… - Molecular Ecology …, 2025 - Wiley Online Library
Mitigating ongoing losses of insects and their key functions (eg pollination) requires tracking
large‐scale and long‐term community changes. However, doing so has been hindered by …

Metagenomics versus total RNA sequencing: most accurate data-processing tools, microbial identification accuracy and perspectives for ecological assessments

CA Hempel, N Wright, J Harvie, JS Hleap… - Nucleic Acids …, 2022 - academic.oup.com
Metagenomics and total RNA sequencing (total RNA-Seq) have the potential to improve the
taxonomic identification of diverse microbial communities, which could allow for the …

Metagenomic analysis using phylogenetic placement—a review of the first decade

L Czech, A Stamatakis, M Dunthorn… - Frontiers in …, 2022 - frontiersin.org
Phylogenetic placement refers to a family of tools and methods to analyze, visualize, and
interpret the tsunami of metagenomic sequencing data generated by high-throughput …

crabs—A software program to generate curated reference databases for metabarcoding sequencing data

GJ Jeunen, E Dowle, J Edgecombe… - Molecular ecology …, 2023 - Wiley Online Library
The measurement of biodiversity is an integral aspect of life science research. With the
establishment of second‐and third‐generation sequencing technologies, an increasing …

Comparative environmental RNA and DNA metabarcoding analysis of river algae and arthropods for ecological surveys and water quality assessment

K Miyata, Y Inoue, Y Amano, T Nishioka, T Nagaike… - Scientific Reports, 2022 - nature.com
Environmental DNA (eDNA) metabarcoding is widely used for species analysis, while the
use of environmental RNA (eRNA) metabarcoding is more limited. We conducted …

Coming of age for COI metabarcoding of whole organism community DNA: towards bioinformatic harmonisation

TJ Creedy, C Andújar… - Molecular Ecology …, 2022 - Wiley Online Library
Metabarcoding of DNA extracted from community samples of whole organisms (whole
organism community DNA, wocDNA) is increasingly being applied to terrestrial, marine and …

COInr and mkCOInr: Building and customizing a nonredundant barcoding reference database from BOLD and NCBI using a semi‐automated pipeline

E Meglécz - Molecular Ecology Resources, 2023 - Wiley Online Library
Reference databases with wide taxonomic coverage are greatly needed in many fields of
biology, most particularly for the taxonomic assignment of metabarcoding sequences …