Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins
One of the most important branches of genetic engineering is the expression of recombinant
proteins using biological expression systems. Nowadays, different expression systems are …
proteins using biological expression systems. Nowadays, different expression systems are …
Yeast expression systems: overview and recent advances
Yeasts are outstanding hosts for the production of functional recombinant proteins with
industrial or medical applications. Great attention has been emerged on yeast due to the …
industrial or medical applications. Great attention has been emerged on yeast due to the …
CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris
Q Liu, X Shi, L Song, H Liu, X Zhou, Q Wang… - Microbial cell …, 2019 - Springer
Abstract Background Pichia pastoris (syn. Komagataella phaffii) is a widely used generally
recognized as safe host for heterologous expression of proteins in both industry and …
recognized as safe host for heterologous expression of proteins in both industry and …
Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production
WC Liu, S Inwood, T Gong, A Sharma… - Critical Reviews in …, 2019 - Taylor & Francis
Pichia pastoris is extensively used to produce various heterologous proteins. Amounts of
biopharmaceutical drugs and industrial enzymes have been successfully produced by fed …
biopharmaceutical drugs and industrial enzymes have been successfully produced by fed …
Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain–based vaccine candidate, RBD219-N1C1
Abstract A SARS-CoV-2 RBD219-N1C1 (RBD219-N1C1) recombinant protein antigen
formulated on Alhydrogel® has recently been shown to elicit a robust neutralizing antibody …
formulated on Alhydrogel® has recently been shown to elicit a robust neutralizing antibody …
Impact of extraction techniques and process optimization on antioxidant and antibacterial potential of Kalanchoe pinnata leaf extract
ABSTRACT Kalanchoe pinnata (Lam.) Pers. is an ethnomedicinal plant known for its
antioxidant, antibacterial, and therapeutic potential. The current study was an effort to …
antioxidant, antibacterial, and therapeutic potential. The current study was an effort to …
Non-conventional yeast cell factories for sustainable bioprocesses
The non-conventional yeasts Kluyveromyces lactis, Yarrowia lipolytica, Ogataea
polymorpha and Pichia pastoris have been developed as eukaryotic expression hosts …
polymorpha and Pichia pastoris have been developed as eukaryotic expression hosts …
Advances in Komagataella phaffii Engineering for the Production of Renewable Chemicals and Proteins
CVGC Carneiro, LA Serra, TF Pacheco, LMM Ferreira… - Fermentation, 2022 - mdpi.com
The need for a more sustainable society has prompted the development of bio-based
processes to produce fuels, chemicals, and materials in substitution for fossil-based ones. In …
processes to produce fuels, chemicals, and materials in substitution for fossil-based ones. In …
Escherichia coli and Pichia pastoris: microbial cell-factory platform for full-length IgG production
Owing to the unmet demand, the pharmaceutical industry is investigating an alternative host
to mammalian cells to produce antibodies for a variety of therapeutic and research …
to mammalian cells to produce antibodies for a variety of therapeutic and research …
Enhancing the Heterologous Expression of a Thermophilic Endoglucanase and Its Cost-Effective Production in Pichia pastoris Using Multiple Strategies
W Dai, H Dong, Z Zhang, X Wu, T Bao, L Gao… - International Journal of …, 2023 - mdpi.com
Although Pichia pastoris was successfully used for heterologous gene expression for more
than twenty years, many factors influencing protein expression remain unclear. Here, we …
than twenty years, many factors influencing protein expression remain unclear. Here, we …