Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live
specimens. We review recent technical advances in SR-SIM, with emphasis on imaging …

Here we describe a protocol for advanced CUBIC (Clear, Unobstructed Brain/Body Imaging
Cocktails and Computational analysis). The CUBIC protocol enables simple and efficient …

Super-resolution microscopy (SRM) is a fast-develo** field that encompasses
fluorescence imaging techniques with the capability to resolve objects below the classical …

Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields
have been combined only recently to facilitate novel experiments and measurements in …

Super-resolution microscopy can unravel previously hidden details of cellular structures but
requires high irradiation intensities to use the limited photon budget efficiently. Such high …

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

CLARITY is a method for chemical transformation of intact biological tissues into a hydrogel-
tissue hybrid, which becomes amenable to interrogation with light and macromolecular …

We report a three-dimensional microscopy technique—swept, confocally-aligned planar
excitation (SCAPE) microscopy—that allows volumetric imaging of living samples at …