High-throughput screening, next generation sequencing and machine learning: advanced methods in enzyme engineering

R Vanella, G Kovacevic, V Doffini… - Chemical …, 2022 - pubs.rsc.org
Enzyme engineering is an important biotechnological process capable of generating
tailored biocatalysts for applications in industrial chemical conversion and biopharma …

Recent advances of metabolic engineering strategies in natural isoprenoid production using cell factories

M Li, F Hou, T Wu, X Jiang, F Li, H Liu, M **an… - Natural Product …, 2020 - pubs.rsc.org
Covering: up to 2019 As abundant natural products, isoprenoids have many useful industrial
applications in the manufacturing of drugs, fragrances, food additives, colorants, rubber and …

Machine-learning guided elucidation of contribution of individual steps in the mevalonate pathway and construction of a yeast platform strain for terpenoid production

M Mukherjee, RH Blair, ZQ Wang - Metabolic engineering, 2022 - Elsevier
The production of terpenoids from engineered microbes contributes markedly to the
bioeconomy by providing essential medicines, sustainable materials, and renewable fuels …

Potential natural food preservatives and their sustainable production in yeast: terpenoids and polyphenols

X Lyu, J Lee, WN Chen - Journal of agricultural and food chemistry, 2019 - ACS Publications
Terpenoids and polyphenols are high-valued plant secondary metabolites. Their high
antimicrobial activities demonstrate their huge potential as natural preservatives in the food …

Recent Advances and Multiple Strategies of Monoterpenoid Overproduction in Saccharomyces cerevisiae and Yarrowia lipolytica

DX Li, Q Guo, YX Yang, SJ Jiang, XJ Ji, C Ye… - ACS Synthetic …, 2024 - ACS Publications
Monoterpenoids are an important subclass of terpenoids that play important roles in the
energy, cosmetics, pharmaceuticals, and fragrances fields. With the development of …

Metabolic engineering of microbes for monoterpenoid production

K Zhu, J Kong, B Zhao, L Rong, S Liu, Z Lu… - Biotechnology …, 2021 - Elsevier
Monoterpenoids are an important class of natural products that are derived from the
condensation of two five‑carbon isoprene subunits. They are widely used for flavouring …

Plasticity engineering of plant monoterpene synthases and application for microbial production of monoterpenoids

D Lei, Z Qiu, J Qiao, GR Zhao - Biotechnology for biofuels, 2021 - Springer
Plant monoterpenoids with structural diversities have extensive applications in food,
cosmetics, pharmaceuticals, and biofuels. Due to the strong dependence on the …

Metabolic engineering strategies to overcome precursor limitations in isoprenoid biosynthesis

Y Zu, KLJ Prather, G Stephanopoulos - Current Opinion in Biotechnology, 2020 - Elsevier
Highlights•Enhancing precursor supply is effective for improving isoprenoid
biosynthesis.•Both local and global approaches relative to the biosynthetic pathway are …

A well‐characterized polycistronic‐like gene expression system in yeast

M Mukherjee, ZQ Wang - Biotechnology and Bioengineering, 2023 - Wiley Online Library
Efficient expression of multiple genes is critical to yeast metabolic engineering for the
bioproduction of bulk and fine chemicals. A yeast polycistronic expression system is of …

The construction and optimization of engineered yeast chassis for efficient biosynthesis of 8‐hydroxygeraniol

Y Zhang, M Yuan, X Wu, Q Zhang, Y Wang, L Zheng… - Mlife, 2023 - Wiley Online Library
Microbial production of monoterpenoid indole alkaloids (MIAs) provides a sustainable and
eco‐friendly means to obtain compounds with high pharmaceutical values. However …