Controlling and enhancing CRISPR systems
Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins
CRISPR loci and Cas proteins provide adaptive immunity in prokaryotes against invading
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing
CRISPR-Cas13 proteins are RNA-guided RNA nucleases that defend against incoming
RNA and DNA phages by binding to complementary target phage transcripts followed by …
RNA and DNA phages by binding to complementary target phage transcripts followed by …
RNA targeting unleashes indiscriminate nuclease activity of CRISPR–Cas12a2
Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-
nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded …
nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded …
Anti-CRISPRs: protein inhibitors of CRISPR-Cas systems
Clustered regularly interspaced short palindromic repeats (CRISPR) together with their
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …
AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation
W Sun, Z Cheng, J Wang, J Yang, X Li… - Proceedings of the …, 2023 - National Acad Sciences
Anti-CRISPR (Acr) proteins are encoded by phages and other mobile genetic elements and
inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum …
inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum …
[HTML][HTML] A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression
Used widely for genome editing, CRISPR-Cas enzymes provide RNA-guided immunity to
microbes by targeting foreign nucleic acids for cleavage. We show here that the native …
microbes by targeting foreign nucleic acids for cleavage. We show here that the native …
Novel bacterial clade reveals origin of form I Rubisco
Rubisco sustains the biosphere through the fixation of CO2 into biomass. In plants and
cyanobacteria, form I Rubisco is structurally comprised of large and small subunits, whereas …
cyanobacteria, form I Rubisco is structurally comprised of large and small subunits, whereas …
Machine learning predicts new anti-CRISPR proteins
The increasing use of CRISPR–Cas9 in medicine, agriculture, and synthetic biology has
accelerated the drive to discover new CRISPR–Cas inhibitors as potential mechanisms of …
accelerated the drive to discover new CRISPR–Cas inhibitors as potential mechanisms of …
Structures and strategies of anti-CRISPR-mediated immune suppression
More than 50 protein families have been identified that inhibit CRISPR (clustered regularly
interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we …
interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we …